In august 2017, symptoms of leaf spot disease were found in okra (Abelmoschus esculentus L.) cultivated area of university of Rajshahi. Several morphological and biochemical tests were conducted for the identification and characterization of the causal agent of leaf spot disease of okra. The pathogen was isolated from infected leaf and morphological identification initially confirms the presence of the fungi of Cercospora spp. For the growth profiling, the fungi was grown in different media, temperature, pH, sugar concentrations, acid concentrations (Citric, Malic and Nicotinic Acid) and different carbohydrates (sucrose, fructose, maltose, glucose, lactose). The optimum growth of the fungi was observed at 25⁰C, pH 6 and 20% sugar concentration. The highest 43±0.3 mm radial colony growth was observed in Sucrose and Maltose with 0.457 gm and 0.435 gm dry weight. The fungi could not grow at 2% concentration of citric and malic acid. Cellulolytic activity was not observed for this strain. A BlastN search of 511bp obtained sequence was performed in GenBank database. And it revealed that, the strains had approximately 90% identity with several Cercospora spp. The obtained sequence deposited in GenBank (accession No. MG735450).

Introduction: With every unit of blood there is 1% chance of transfusion associated problems including transfusion transmitted infections (TTI). These TTIs include hepatitis B (HBV), hepatitis C (HCV), Human Immunodeficiency Virus (HIV), syphilis, and less commonly malaria, toxoplasmosis, brucellosis, other viral infections. The process of preventing the transmission of TTIs through blood transfusion presents one of the greatest challenges of transfusion medicine. Aim: The aim of our study was to find out the seroprevalence of infectious markers of TTIs among the blood donors. Materials and methods: A 9 year retrospective study was carried out in the blood bank of KIMS, Hubballi, Karnataka, from January 2007 to December 2015. From the donor’s blood units, 5 ml blood samples were obtained for serological testing. All the samples were screened for HIV, HCV, HBV by ELISA and RPR for syphilis. Results: A total of 80312 apparently healthy donors were analyzed for prevalence of TTIs of which 50735 (63.17%) were replacement donors and 29577 (36.83%) were voluntary donors. The overall prevalence of TTIs was 2047/80312 (2.5 %). The overall prevalence of HIV, HBV, HCV and syphilis among donors were 283(0.35%), 1556 (1.9%), 195 (0.2%) & 13(0.01%) respectively. Conclusion: The current infectious disease pattern and trends in donor population can help in planning of future blood transfusion related health challenges. Encouraging female population as well as voluntary blood donors for blood donation will increase the number of donors and safe donor pool.

The Research study was conducted by Krishi Vigyan Kendra Sawai Madhopur during kharif-2017 under National Mission on Oilseed and Oilpalm (NMOOP) Project to enhancement the productivity and profitability of sesame by transfer of improved technology. The result revealed that the maximum seed yield of sesame was obtained under demonstrated plots ranged between 6.27 q/ha to 6.72 q/ha with an average of 6.45 q/ha which was 25.97 percent higher as compared to local check with an average of 5.12 q/ha. Extension gap between demonstrated plots and local check was found with an average of 1.33 q/ha. The maximum average net returns (Rs. 31190 ha-1) and benefit cost ratio (2.70) were recorded under demonstrated plots as compared to local check (Rs. 22603 ha-1 and 1.87 respectively).

In the present study, generation mean analysis 60 treatments (10P1, 10P2, 10 F1, 10 F2, 10 BC1 and 10 BC2) was chosen to study the nature and magnitude of gene effects for yield and yield attributing traits. analysis of variance was carried out for all the 10 characters. Highly significant difference was observed among treatments for all the characters except days to maturity and number of primary branches per plant. Such observations were also reported. Useful heterosis over better parent and mid parent the best crosses. viz., NPHY 29 X LCK 87312, NP 121 X RR 76 and NPRR 271 X No 3 for seed yield per plant were considered. Inbreeding depression in F2 generation was observed for all characters except number of secondary branches per plant, number of capsules per plant and seed yield per plant. Medium heritability was observed in characters like number of primary branches per plant. In F1 generation characters likes number of seeds per capsule exhibited high value of genetic advance.

Rice (Oryza Sativa L.) is an important food crop. The demand for rice continues to increase owing to continued growth of population. It is predicted that a 50% to 60% increase in rice production will be required to meet demand by 2025 in India. Cow urine (Gomutra) is used for several centuries in different ways in many parts of the world. Cow urine contains nitrogen constituents in different amounts and may be used as source of nitrogen for plant nutrition. Hence, the present study was taken up to evaluate the effect of cow urine on growth yield and nutrient uptake of rice. Accordingly, a field experiment was conducted during kharif 2017at crop cafeteria of BHU- Krishi Vigyan Kendra, Institute of Agricultural Sciences, Rajiv Gandhi South campus, Barkachha, Mirzapur (Banaras Hindu University) in randomized block design taking rice variety Shusk Samrat as a test crop. The soil of the study region was red soil having pH 7.8, EC 0.67 dS m-1, organic carbon 0.42%, available N 185.3, P 9.6, K 189 and S 9.4 kg ha-1and available micronutrient Fe 32.6, Mn 18.6, Zn 2.26, Cu 2.1 and Boron 0.98 mg kg-1. The experiment consisted of five treatments comprising T1 (control- no input), T2 (N at 120 kg ha-1), T3 (N at 150 kg ha-1), T4 (N at 120 kg ha-1 + cow urine) and T5 (N at 100 kg ha-1) taking four replications in randomized block design. Cow urine procured from same cow was applied weekly up to six weeks from the date of transplanting. The results of the study revealed that paddy crop favourably responded to different levels of nitrogen through fertilizer alone and in combination of cow urine. Plant growth (plant height, number of tiller) and yield (grain and straw) significantly increased as compared to control by the application of nitrogen. Application of N at 120 kg ha-1 + cow urine registered maximum dry matter production. Without cow urine, application of nitrogen at 150 kg ha-1 recorded higher yield than control and 120 kg ha-1 level. Nitrogen content in plant (grain and straw) and uptake were also found maximum when nitrogen was applied at 120 kg ha-1 + cow urine.

Present in vitro experiment was carried out to identify plant growth promoting rhizobial isolates for abiotic stress tolerance. Rhizospheric soils from normal, salt affected, drought affected were collected from different places of Telangana state. From all soil samples, twenty one rhizobial isolates were collected. Among the twenty one isolates, eleven isolates were showed different plant growth promoting properties. The results of different in vitro abiotic stress tolerance of isolates reveals that, only one isolate showed tolerance to temperature from 20ºC-50ºC (YR-21), three isolates were showed tolerance to temperature from 20ºC - 45ºC (RR-1, GR-5, GNR-8), one isolate was showed tolerance to temperature from 30ºC- 45ºC (MR-11), only one isolate were showed tolerance to temperature from 30ºC - 40ºC (ASR-17). Two isolates showed tolerance to water potential from - 0.05 Mpa to- 0.15 Mpa (RR-1, RR-3), three isolates were showed tolerance to water potential from - 0.05 Mpa to -0.30 Mpa (GNR-8, ASR-19, YR-21), only one isolate showed tolerance to water potential at - 0.05 Mpa (GNR-7).

Introduction: The alarmingly worsening scenario of Multi drug-resistant tuberculosis (MDR-TB) call for urgent need for a simple method for the rapid detection of drug-resistant TB. In MTB katG mutations are major cause of INH resistance. The usefulness of INH, a key component of short-course chemotherapy of tuberculosis, is threatened by the emergence of drug-resistant strains of MTB with mutations in the katGgene. Objective: This study is an effort to study the frequency of mutations in INH in Western Maharashtra, India. Methods: Samples were processed for two molecular methods, GenoTypeMTBDRplus (LiPA) and Dideoxy Sanger Sequencing. Samples processed for DNA extraction, nested PCR reaction was done by annealing at 550c with specific primers. After confirmation of band on Gel Doc, Sequencing was done with one primer. Sequencing was also done for inhA and inhA promoter region. Result: Major mutation found was S315T i.e. ser is replaced by thr at 315 positions. We could find other mutation at different positions. Conclusion: Molecular tests are rapid and accurate. S315T can be potential genetic marker for isoniazid resistance.

A total of thirty isolates of A.alternata were evaluated for their characteristics at four different temperatures viz., 15, 20, 25and 30oC on potato dextrose agar medium (PDA).The data recorded on colony diameter and rate of growth after incubation at various temperature was found significantly different. Results revealed that most of the isolate had maximum growth at 30°C followed by 25°C. Minimum growth for most of the isolates was recorded in 15°C followed by 20°C. Rate of growth at different temperatures for most of the isolates of A.alternata was also found significantly maximum at 30°C followed by 25°C except few isolates like Pindra, Chirrayyakot, Jamalpur (JJ), Keshavpur, Bhikharipur, Raibarely and Nalanda where growth rate at 25°C was higher than 30°C.

Background: Extended spectrum β-lactamase (ESBL) producing gram negative organisms particularly the multi-drug resistant strains have become a major global health problem. Aims: The present study was aimed at determining the prevalence of extended spectrum β-lactamase (ESBLs) production in gram negative bacterial isolates obtained from various clinical isolates and to detect their antibiotic sensitivity pattern. Material and Methods: A total of 173 gram negative isolates obtained from various clinical specimens were processed by conventional methods and the ESBL detection was done by phenotypic confirmatory disc diffusion test along with the routine susceptibility testing recommended by CLSI. Results: 82 (47.4%) among the 173 gram negative isolates were ESBL producers. The most common ESBL producing organism was E.coli (53.08%). The isolates from pus (59.61%) showed the maximum ESBL production. Carbepenems were found to the most effective drug against ESBL producers. Conclusion: The high rate of ESBL production along with high degree of antibiotic co-resistance among the ESBL strains in our study emphasizes on the need for routine surveillance of ESBL among gram negative isolates using the phenotypic confirmatory test which can help the clinicians in early and effective disease management.

Microalgal samples were collected from different regions of India. The samples thus obtained were serially diluted, colonies were further purified and identified as Desmodesmus abundans, Scendesmus pectinatus, Tetranephris brasiliensis, Desmodesmus armatus, Scendesmus costato-granulatus, Neocystis brevis, Chlorella sorokiniana, Scendesmus acutus, Pseudomuriella species and Chlorella minutissima by 18s rRNA sequencing. The effect of growth conditions was studied under different media such as Bold Basal Media (BBM), Blue Green 11 media (BG11) and Tris-Acetate Phosphate media (TAP). The highest growth rate was occurred in Desmodesmus abundans on 19th day in TAP media whereas the lowest growth rate was occurred in Tetranephris brasiliensis in BBM media. Under similar environmental conditions average specific wet and dry biomass were found maximum in Chlorella minutissima, Desmodesmus abundans and Scendesmus costato-granulatus in TAP media when compared to BBM and BG11. The highest amount of lipid was obtained in Desmodesmus armatus (43.7%) grown in TAP media. Nile red staining and thin layer chromatography confirms the accumulation of total lipids. The reaction condition with that of n-hexane to methanol ratio 1:2, solvent dosage 5.0 ml, reaction temperature 90°C, reaction time 2.0 h and catalyst volume 0.5 ml of the direct transesterification can reach the conversion yield of 90.23%, 89.25% and 86.65% found in Desmodesmus abundans, Desmodesmus armatus and Scendesmus costato-granulatus respectively. There is a prospective in algal biotechnology by exploitation of different media composition for the growth formulation in microalgae species which is inexpensive for biomass cultivation in large scale production of lipids that can be harnessed for biodiesel production.

A total of eleven sugars were evaluated for their potential to endow germination in spores of Bacillus megaterium MTCC 2949. Germination of spores in response to different sugars was studied by two well-known germination assays namely reduction in O.D. (600nm) and refractility examination. As a result, reduction in O.D. (600 nm) varied from ~3.90 to 42.01% depending upon type and concentration of different sugars. Spores were found germinated well with a reduction in absorbance of 30% or more with cellobiose, dextrose, 2-deoxy-D-glucose, glucosamine, maltose, methyl-α-D- glucoside, sorbose, starch and xylose. On the other hand, germination was found at a poor or negligible rate (i.e., < 6% reduction of absorbance) with D-galactose and rhamnose. The finding of this assay was further validated by refractility study using phase contrast microscopy. The outcome of presented work reveals that all sugars are not necessarily capable of inducing germination in bacterial spores and hence induction of germination is linked with the requirement of specific sugars.

Mycobacterium avium subsp paratuberculosis (MAP) is classified under list B transmissible organisms signifying the public health concern and needs specific early diagnosis. Due to the cross reacting antigens of environmental mycobacterium and the diverse immune responses of individuals, cocktail of antigens capable of inducing strong immune response should be identified to improve serum-based methods of detection. Insertional sequence (IS900) was specific to par tuberculosis among the mycobacterium family, and among the IS900 elements, a nir A gene at locus 4 was putative to MAP and absent from the M. avium genome. In an attempt to identify the candidate antigens, nitrate reductase gene nir A was successfully cloned and expressed in E. coli. In Western blot analysis strong immune reactivity was shown by the recombinant protein nitrate reductase with bovine anti MAP antibodies facilitates its use as a diagnostic candidate to improve the specificity of MAP diagnosis.

Introduction: Respiratory infections in critically ill patients are associated with high morbidity and mortality. Patients who are mechanically ventilated are at high risk of acquiring respiratory tract infections due to complex interplay between the endotracheal tube, host immunity and virulence of invading bacteria. To initiate empiric antimicrobial therapy knowledge of local antimicrobial resistance patterns are essential. Material And Methods: A cross sectional study of 48 adult patients who were mechanically ventilated for various reasons in ICU of our hospital from June 2015 to May 2017 was undertaken to study profile and sensitive characteristics of infective organisms from endotracheal aspirate obtained after 48 hours of intubation. Results: Gram negative bacteria (83.02%) were isolated from most of the patients and Gram positive organisms were 16.98%. The most common being Acinetobacter spp. (33.96%), followed by Klebsiella pneumoniaee (32.07%) and Pseudomonas aeruginosa (15.09%). Gram positive cocci i.e. Staphylococcus aureus isolated in (16.98%) patients. Most of these gram negative organisms were susceptible to colistin and tigecycline antibiotics. Most of these gram positive organisms were susceptible to vancomycin and linezolid. Conclusion: Gram negative organisms susceptible to colistin, tigecycline and meropenem antibiotics form the predominant isolates in our critical care setup. A local antibiogram for each hospital, based on bacteriological patterns and susceptibilities is essential to initiate empiric therapy, to prevent poor outcomes and help in framing the appropriate institutional antibiotic policy.

Group A rotavirus leftovers a main reason of diarrhea in infants and young children particularly in developing countries. Nursing changes in rotavirus strains is essential to measure the possible effectiveness of vaccines in exact geographic sites. Stool samples were collected from children below 2 years suffering after severe diarrhea. Positive samples were amplified to examine and characterized VP4 gene. The greatest predominant genotype was G1P[8] (6/13)(46.15) followed by G3P[8] (2/13)(15.38), G2P[6] (1/13)(7.69), G4P[8] (1/13)(7.69), G9P[8] (1/13)(7,69). A phylogenetic analysis and sequencing identity matrix to VP4 gene of isolates detected in the present study exposed that G1P8 (Iq4-f2,Iq4-f5, Iq4-f7, Iq4-f6, Iq4-f4) in lineage 1 of phylogenetic tree, similarity between those isolates 100%. G1P8 (Iq4-s3) and G4P8 (Iq4-f1) of lineage 1 similarity between these isolates (94%), and isolates Iq4-s3 and Iq4-f1 identity (93-94%) to next isolates (Iq4-f2, Iq4-f5, Iq4-f7, Iq4-f6, Iq4-f4). Two isolates (Ig4-f8, Ig4-f9) in lineage 2 was similar (99%). Vaccine isolate of vp4 gene (lineage 1) similarity (89%) to G4P8 (Iq4-f1), (88%) to G1P8 (Iq4-f2, Iq4-f4, Iq4-f5, Iq4-f6, Iq4-f7). The distribution of genotypes that own neither VP4 specificity through the obtainable rotavirus vaccine presently in use may embody a challenge to the consequence and accomplishment of vaccination.

Twenty three endophytic bacterial isolates were isolated from the roots of Salvadora persica L. were characterized on the basis of various criteria such as morphology and biochemical characteristics, out of which six potential endophytes were selected based on their PGPR activity and identified by 16S rRNA gene sequence analysis as Citrobacter sp. A6 (KY587407), Pantoea agglomerans A10 (KY587408), Pseudomonas oryzihabitans A16 (KY963571), Serratia marcescens A20 (KY963572) Enterobacter aerogenes A23 (KY963573) and Bacillus sp. A26 (KY963574). Only Citrobacter sp. A6 (KY587407), Pantoea agglomerans A10 (KY587408) were able to produce IAA. Siderophore production was observed in only Enterobacter aerogenes A23. All isolates solubilized tricalcium phosphate except Bacillus sp. A26 and ACC deaminase production were observed in Citrobacter sp. A6 and Bacillus sp. A26. All isolates could withstand higher salt level (7 % NaCl) whereas Serratia marcescens A20 tolerated up to 5 % of NaCl.

Introduction: The genus Trichosporon usually cause superficial mycoses, but recently they have emerged as an important pathogen causing invasive infections in immuno-compromised patients. Invasive Trichosporonosis is mainly documented in patients with associated risk factors and co-morbid conditions like haematological malignancies, cardiac or renal disease, intravenous or central line catheter, prosthetic devices and implants, prior antibiotic therapy, stay in Intensive care unit. Objective: This study was undertaken to assess the risk factors and co-morbid conditions associated with Trichosporonosis and its clinical outcome. Materials and Methods: We considered around 72 clinical isolates for the study that were, dry yeast like colonies, urease positive, showed blastoconidia and arthroconidia in gram stain, and were suspected to belong to the genus Trichosporon. Later, they were confirmed genotypically using Trichosporon specific PCR. The demographic and clinical details were collected from all the patients for the study. Results: About 66% of the affected patients were males, with male to female ratio 2:1. All our patients had any one of associated risk factor. Indwelling catheter was present in most of the patients (97.2%) in our study. Bladder catheterisation was the predominant risk factor among patients who grew Trichosporon sp. in urine sample (83.7%). Majority of the patients (52.8%) had associated bacterial infections. Discussion and conclusion: The associated risk factors increase the colonisation of this genus by breaking mucosal barrier, later leading to establishment of infection.

The present manuscript deals with the synthesis and characterization of some novel organobismuth derivatives of isatin for their antimicrobial activity. These compounds were synthesized by the reported method and characterized with the help of M.P., elemental, I.R spectral analysis along with their efficacy against pathogenic bacterial and fungal strains. It was found that the compounds exhibit higher activity than the standard drug for bactericidal and fungicidal activity.

The Present Manuscript deals the synthesis of some novel organobismuth(III)aryloxyacetates followed by their characterization by melting points, elemental analysis, U.V., I.R and NMR spectral analysis to confirm their structures and geometry. The compounds were also screened for their antibacterial and antifungal efficacy against pathogenic bacterial and fungal strains. It was found that these compounds exhibit higher activity than the standard drug for bactericidal and fungicidal activity.

Studies were carried out to establish an efficient system for in vitro regeneration of sugarcane cultivar, 2003V46 using chemical mutagens; Ethyl methane sulphonate (EMS) and 2,4dichlorophenoxy acetic acid (2,4D). Young leaf rolls were used as explants for callus induction on MS medium containing different concentrations of EMS(0.6 µM l-1, 0.8 µM l-1 and 1.0 µM l-1) and 2,4D(4mg l-1, 5mg l-1 and 6 mg l-1). Among the different concentrations,2003V46 had taken minimum number of days (10.0) for callus induction with 2, 4D @ 4mg l-1,recordingmaximum mean callus size of 2.42 cm with high callus induction frequency (96.89%) and maximum mean number of explants inducing callus (2.86) in addition with superior response in shooting, rooting and hardening characters than EMS.2, 4D at higher concentration (6 mg l-1) had inhibitory effect on callusing, shooting and rooting and produced variation for leaf sheath hairiness, shape of bud and internode colour similar to that of the mutagen, EMS.

Aim: The study was carried out with aim to isolate methicillin resistant staphylococci from milk samples and determine antibiogram pattern of methcillin resistant staphylococcal isolates. Materials and Methods: A total of 50 milk samples were collected aseptically from different places in and around Junagadh city. Which were subjected to cultural isolation and biochemical characterization with routine and MRSA specific media, Kirby-Bauer disk diffusion method were utilized for antibiogram. Results: Results showed that out of total 50 samples, 7 isolates (14%) were found to be MRS. The MRS isolates showed highest sensitivity towards chloramphenicol (100%), gentamicin (85.71%), ampicillin/sulbactam (71.42%), oxytetracyline (71.42 %), levofloxacin, enrofloxacin and ceftriaxone (57.14 %). The pattern clearly indicated that the overall high percent of MRS isolates were resistant to penicillin-G (100 %), methicillin (100%), amoxicillin/sulbactam (100 %) followed by cefotaxime/clavulanic acid (85.71 %), amoxyclav (71.42 %), ceftriaxone/sulbactam and cefoperazone/sulbactam (57.14 %). Conclusions: Staphylococcus spp. was found to be the major cause of bovine mastitis in this geographical region and methicillin resistant isolates from milk was showing multidrug resistant.

In the present study generation mean analysis 60 treatments (10P1, 10P2, 10 F1, 10 F2, 10 BC1 and 10 BC2) was chosen to study the nature and magnitude of gene effects for 8 quality traits. analysis of variance was carried out for 8 quality traits. Highly significant difference observed among treatments, for all the characters except protein content (%) and stearic acid. Highly significant difference observed among parents, for all the characters except seed yield per plant (g), protein content (%) and palmitic acid. Highly significant difference observed among F1s, for all the characters except oil content (%), stearic acid, oleic acid, linoleic acid and linolenic acid. Highly significant difference observed among parent vs crosses, for all the characters except protein content (%). Useful heterosis over better parent and mid parent the best crosses. viz., NPRR271 X NO 356 and RL-28-1 X L-53 for oil content (%) were considered. Medium heritability was observed in characters like palmitic acid, linoleic acid and linolenic acid, whereas low heritability was observed in characters like seed yield per plant (g), oil content (%), protein content (%), stearic acid and oleic acid. In F1 generation characters likes palmitic acid and oleic acid exhibited high value of genetic advance.

Dairy is one of the major industries causing water pollution with its large water consumption. In present study cyanobacterial species were isolated from dairy industries effluent and explored for removal of nutrients from dairy effluent simultaneously biomass produced again used for extraction of high value compounds. The percentage of nitrogen (N) and phosphate (P) reduction was 78.12%, 88.91%, 78.66% and 87.33%, 79.68%, 78.57% by spirulina, oscillatoria species and its consortium, respectively after 18 days. In case of high value compounds maximum amount of C-phycocyanin 0.431 mg/L and phycoerythrin 0.165 mg/L was extracted from oscillatoria species. The purification of the crude extract done by ammonium sulfate fractionation and Sephadex-G50 size exclusion chromatography. The lipid content from oscillatoria species was about 125.65 mg/l-1 which can be used for biofuel production.

Degradation of coffee pulp waste is time taken process. The microorganisms isolated from coffee pulp waste could be used as catalysts in the biodegradation of pulp waste which reduces the composting period. An experiment was conducted in which coffee pulp waste was collected from Central Coffee Research Institute farm and isolated two Aspergillus spp. (Asp.1 and Asp.2) two Trichoderma spp. (Tri.1 and Tri.2) and two-gram negative bacteria (CPB-1 and CPB-2). These isolated microbes were subjected to pectinolytic enzyme activity assay by plate assay method. Clear zone of pectinolytic enzyme activity was observed in Aspergillus spp., Trichoderma spp. and CPB-1 bacterium isolate whereas clear zone was not observed in CPB-2 bacterium isolate.

Rajasthan ranked first among all states of India in terms of both area and production of rapeseed and mustard so there is no doubt to say mustard state to Rajasthan in spite of having a lot of technology, production of mustard is not up to the higher extent. Keeping this view, study was conducted in four gram panchayats of Tonk district, Rajasthan.An exhaustive list of mustard growers from selected villages were prepared and total 130 respondents were selected randomly through proportional allocation method. The study revealed that the majority of the farmers (92) were found to have medium knowledge level (70.77 per cent) whereas 16.15 per cent and 13.08 per cent of farmers were having high and low knowledge level, respectively about recommended production technology of rapeseed and mustard crop. It was also found that the respondents possessed maximum knowledge regarding “seed rate and recommended spacing” and “Time of sowing” of rapeseed and mustard production technology, respectively. Similarly, they possessed less knowledge regarding “seed treatment” and “plant protection measures”, respectively.

A cross sectional study involving 97 dentists was conducted from January to December 2006. All professionals gave written consent for the procedure and filled out a questionnaire about some risk factors. Blood samples were analyzed to detect the serological markers: HBsAg, anti-HBs, anti-HBc and anti-HCV. A total of 59 (60.8%) dentists showed seropositivity for at least one of the viral markers to HBV. The presence of total anti-HBc was observed in 1 (1.0%) of the dentists. None of them was HBsAg, 5 (5.2%) were anti-HBc/anti-HBs positive and 53 (54.6%) dentists were anti-HBs positive. Anti-HCV seropositivity was 3.1% (3/97) and all the subjects had more than 25 years of experience in the profession. The HBV and HCV seropositivity rates among dentists of Belém, Pará was similar to that detected in Brazil and the low HBV vaccination coverage shows that there is the necessity of performing the vaccination scheme in this populationd.