ISOLATION AND IDENTIFICATION OF METHICILLIN RESISTANT STAPHYLOCOCCI FROM MILK SAMPLES WITH BOVINE MASTITIS AND THEIR DRUG RESISTANCE PATTERNS IN AND AROUND JUNAGADH, GUJARAT

H.M. JIVANI¹*, B.S. MATHAPATI², B.B. JAVIA³, V.A. KALARIYA⁴, T.M. SHAH⁵, V.R. NIMAVAT⁶, D.B. BARAD⁷, S.N. GHODASARA^8
1Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
²Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
³Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
⁴Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
⁵Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
⁶Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
⁷Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
⁸Department of Veterinary Microbiology, College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat, India
* Corresponding Author : hetaljivani33@gmail.com

Received : 09-08-2017     Accepted : 28-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1094 - 1096
Int J Microbiol Res 10.3 (2018):1094-1096
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1094-1096

Keywords : Antibiogram pattern, Bovine mastitis, MRS
Academic Editor : Mahajan V., Dr Rahul Singh Arya, Dr Rout
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to College of Veterinary Science and Animal Husbandry, Junagadh Agricultural University, Junagadh 362001, Gujarat
Author Contribution : All author equally contributed

Aim: The study was carried out with aim to isolate methicillin resistant staphylococci from milk samples and determine antibiogram pattern of methcillin resistant staphylococcal isolates. Materials and Methods: A total of 50 milk samples were collected aseptically from different places in and around Junagadh city. Which were subjected to cultural isolation and biochemical characterization with routine and MRSA specific media, Kirby-Bauer disk diffusion method were utilized for antibiogram. Results: Results showed that out of total 50 samples, 7 isolates (14%) were found to be MRS. The MRS isolates showed highest sensitivity towards chloramphenicol (100%), gentamicin (85.71%), ampicillin/sulbactam (71.42%), oxytetracyline (71.42 %), levofloxacin, enrofloxacin and ceftriaxone (57.14 %). The pattern clearly indicated that the overall high percent of MRS isolates were resistant to penicillin-G (100 %), methicillin (100%), amoxicillin/sulbactam (100 %) followed by cefotaxime/clavulanic acid (85.71 %), amoxyclav (71.42 %), ceftriaxone/sulbactam and cefoperazone/sulbactam (57.14 %). Conclusions: Staphylococcus spp. was found to be the major cause of bovine mastitis in this geographical region and methicillin resistant isolates from milk was showing multidrug resistant.

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