J.H. KABARIYA1, V.M. RAMANI2*
1Department of Dairy Microbiology, College of Dairy Science, Kamdhenu University, Amreli, 365601, Gujarat, India
2Department of Dairy Microbiology, College of Dairy Science, Kamdhenu University, Amreli, 365601, Gujarat, India
* Corresponding Author : vimalmramani@gmail.com
Received : 23-03-2018 Accepted : 28-03-2018 Published : 30-03-2018
Volume : 10 Issue : 3 Pages : 1104 - 1108
Int J Microbiol Res 10.3 (2018):1104-1108
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1104-1108
Keywords : Dairy effluent treatment, Cyanobacteria, C-phycocyanin, Phycoerythrin and Lipid content
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Kamdhenu University, Amreli, 365 601, Gujarat, India
Author Contribution : All author equally contributed
Dairy is one of the major industries causing water pollution with its large water consumption. In present study cyanobacterial species were isolated from dairy industries effluent and explored for removal of nutrients from dairy effluent simultaneously biomass produced again used for extraction of high value compounds. The percentage of nitrogen (N) and phosphate (P) reduction was 78.12%, 88.91%, 78.66% and 87.33%, 79.68%, 78.57% by spirulina, oscillatoria species and its consortium, respectively after 18 days. In case of high value compounds maximum amount of C-phycocyanin 0.431 mg/L and phycoerythrin 0.165 mg/L was extracted from oscillatoria species. The purification of the crude extract done by ammonium sulfate fractionation and Sephadex-G50 size exclusion chromatography. The lipid content from oscillatoria species was about 125.65 mg/l-1 which can be used for biofuel production.
1. Vourch M., Balannec B., Chaufer B. and Dorange G. (2008) Desalination, 219, 190–202.
2. Oswald W. J. (2003) Journal of Applied Phycology, 99-106.
3. Blok J., De Morsier A. and Gerike P. (1985) Chemosphere, 14 (11-12), 1805-1820.
4. Sara P. C., Iris A. H., Diana L. C., Nancy O. S., Miguel A., Romero O. and Roberto P. S. (2015) Microbial Biotechnology, 8(2), 190–209.
5. Ferris M. J. and Hirsch C. F. (1991) Appl. Environ. Microbiol, 57, 1448–1452.
6. Elango V., Yuvakkumar R., Jegan S., Kannan N. and Rajendran V. (2008) Advanced Biotech., 23-24.
7. Nancy S. and Mai-Linh H. (2011) Agriculture and Agri-Food Canada Agri-Environment Services Branch.
8. Lenore S. C., Greenberg A. E and Eaton A. D. (1998) APHA 20th Edition, American Public Health Association, NW, Washington, DC.
9. Trivedy R. K. and Goel P. K. (1984) Environmental Publication, Karad.
10. Woertz A., Feffer T. and Lundquist N. Y. (2005) Ph.D. Thesis.
11. Patel A., Mishra S., Pawar R. and Ghosh P. K. (2005) Protein Expression and purification, 40, 248 - 255.
12. Bligh E. G. and Dyer W. J. (1959) Can. J. Biochem. Physiol., 37, 911- 917.
13. Pathade K. N. (2012) An International Refreed & Indexed Quarterly Journal, 2(2), 54-57.
14. Munawar M. (1970a) The Biotype. Hydrobiologia, 35(1), 127-162.
15. Munawar M. (1970b) The Biotype. Hydrobiologia, 36(1), 105-128.
16. Uslu L., Isik O., Koc K., Goksan T. (2011) Afr. J. Biotechnology, 10, 386–389.
17. Lee J. Y., Chan Y., Jun S. Y., Ahn C. Y. and Oh H. M. (2010) Bioresource Technology, 101, S75 – S77.