FREQUENCY OF MUTATION IN ISONIAZID RESISTANT ISOLATES OF Mycobacterium tuberculosis COMPLEX FROM WESTERN MAHARASHTRA INDIA

SWARUPA M. HATOLKAR¹, RABINDRA NATH MISRA²*, NAGESWARI R. GANDHAM³, KALPANA ANGADI⁴, SAVITA V. JADHAV⁵*, NEETU GUPTA^6
1Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
²Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
³Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
⁴Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
⁵Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
⁶Dr D. Y. Patil Medical College, Hospital and Research Centre, Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018
* Corresponding Author : patilsv78@gmail.com

Received : 16-01-2018     Accepted : 10-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1043 - 1045
Int J Microbiol Res 10.3 (2018):1043-1045
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1043-1045

Keywords : MTB, katG, Isoniazide mutations, Genetic marker S315T
Academic Editor : Dr Jalal Ali Bilal, Dr Sourav Sharma, Dr Abheepsa Mishra
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to Dr D. Y. Patil Vidyapeeth, Pimpri, Pune 411018. Author also thankful to geneOm biotechnologies Pvt. ltd, Pune Author highly grateful to technical support of Swati Bhirange for isolation, Yashawant Chavan and Sharad Pawar for sequencing and analysis.
Author Contribution : All author equally contributed

Introduction: The alarmingly worsening scenario of Multi drug-resistant tuberculosis (MDR-TB) call for urgent need for a simple method for the rapid detection of drug-resistant TB. In MTB katG mutations are major cause of INH resistance. The usefulness of INH, a key component of short-course chemotherapy of tuberculosis, is threatened by the emergence of drug-resistant strains of MTB with mutations in the katGgene. Objective: This study is an effort to study the frequency of mutations in INH in Western Maharashtra, India. Methods: Samples were processed for two molecular methods, GenoTypeMTBDRplus (LiPA) and Dideoxy Sanger Sequencing. Samples processed for DNA extraction, nested PCR reaction was done by annealing at 550c with specific primers. After confirmation of band on Gel Doc, Sequencing was done with one primer. Sequencing was also done for inhA and inhA promoter region. Result: Major mutation found was S315T i.e. ser is replaced by thr at 315 positions. We could find other mutation at different positions. Conclusion: Molecular tests are rapid and accurate. S315T can be potential genetic marker for isoniazid resistance.

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