MICROBES FROM COFFEE PULP WASTE AND THEIR PECTINOLYTIC ENZYME ACTIVITY FOR BIODEGRADATION

SANTOSHREDDY MACHENAHALLI¹*, A.P. RANJINI², MADHU S. GIRI³, M. SUDHA⁴, S. DAIVASIKAMANI^5
1Division of Plant Pathology, Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka, India
²Division of Plant Pathology, Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka, India
³Division of Plant Pathology, Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka, India
⁴Division of Plant Pathology, Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka, India
⁵Division of Plant Pathology, Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka, India
* Corresponding Author : santosh3881@gmail.com

Received : 23-03-2018     Accepted : 28-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1109 - 1110
Int J Microbiol Res 10.3 (2018):1109-1110
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1109-1110

Keywords : Coffee pulp waste, Pectinolytic enzyme, Aspergillus spp., Trichoderma spp
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Central Coffee Research Institute, C. R. Station, 577117, Chikkamagaluru, Karnataka
Author Contribution : All author equally contributed

Degradation of coffee pulp waste is time taken process. The microorganisms isolated from coffee pulp waste could be used as catalysts in the biodegradation of pulp waste which reduces the composting period. An experiment was conducted in which coffee pulp waste was collected from Central Coffee Research Institute farm and isolated two Aspergillus spp. (Asp.1 and Asp.2) two Trichoderma spp. (Tri.1 and Tri.2) and two-gram negative bacteria (CPB-1 and CPB-2). These isolated microbes were subjected to pectinolytic enzyme activity assay by plate assay method. Clear zone of pectinolytic enzyme activity was observed in Aspergillus spp., Trichoderma spp. and CPB-1 bacterium isolate whereas clear zone was not observed in CPB-2 bacterium isolate.

1. Adams M.R. and Dougan J. (1981) Trop Sci, 123, 178-196.
2. Aneja K.R. (2012) Experiments in Microbiology, Plant Pathology and Biotechnology. New Age International (P) limited, New Delhi. pp. 607.
3. Barnett H.L. (1960) Illustrated genera of imperfect fungi, second edition, Burgees publishing company, Minneapolis, Minn. pp.52 and 62.
4. Braham J.E. and Bressani R. (1979) Coffee pulp: Composition, technology and utilization. Published by International Development Research Centre, Ottawa, Canada, p. 9.
5. Bressani R. (1979a) Potential uses of coffee berry by-products. In: Coffee pulp: Composition, technology and utilization, publication 108, edited by Braham J. E. and Bressani R. (International Development Research Centre, Ottawa, Canada), 14.
6. Bressani R. (1979b) Anti-physiological factors in coffee pulp. In:Coffee pulp: Composition, technology and utilization, publication 108, edited by Braham, J. E. and Bressani R. (International Development Research Centre, Ottawa, Canada), 83-88.
7. Buerge I.J., Poiger T., Muller M.D. and Buser H.R. (2003) Environ Sci Technol, 37, 691-700.
8. Bui B.H. (2014) Int. J. Recycl. Org. Waste. Agri., 3, 48.
9. Damodaran A. (1978) Indian Coffee, 62, 22-25.
10. Divakaran J. and Elango R. (2009) Glob. J. Env. Res., 3 (2), 92-95.
11. Friedman J. and Waller G. R. (1983) J. Chem. Ecol, (9), 1099-1106.
12. Jyotsna K.P., Vijayalakshmi K., Prasanna N.D. and Shaheen S.K. (2010) Int. J. Life. Sci., 6, 325–327.
13. Opatokun S.A., Kabbashi N., Alam M.Z.,Salihu A.,Abass A. andRuqayyah T.L.D. (2011) Afr. J. Biotechnol. 10:18800–18806.
14. Roussos S., Hannibal L., Aquiahuatla M. A., Hernandez T. M. R. and Marakis S. (1994) J. Food. Sci. Technol., 31,316-319.
15. Soares M.N. C., Da Silva and Gomes R. (1999) Rev. Microbiol., 30, 299-303.