SCREENING AND CHARACTERIZATION OF STRESS TOLERANT RHIZOBIA FROM DIFFERENT SOILS OF TELANGANA

K. DAMODARA CHARI¹*, R. SUBHASH REDDY², S. TRIVENI³, N. TRIMURTULU⁴, V. DURGA RANI⁵, M. SREEDHAR^6
1Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
²Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
³Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
⁴Biofertilizer Production Laboratory, Agricultural Research Station, Amaravathi, Guntur, 522020, Andhra Pradesh, India
⁵Department of Molecular Biology & Biotechnology, Institute of Biotechnology, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
⁶Quality Control Laboratory, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
* Corresponding Author : charigorukallu@gmail.com

Received : 05-03-2018     Accepted : 10-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1038 - 1042
Int J Microbiol Res 10.3 (2018):1038-1042
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1038-1042

Keywords : Rhizobium, PGPR, temperature, drought tolerance
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana. Author acknowledge the financial support extended by the University Grants Commission for National fellowship year 2016-17
Author Contribution : All author equally contributed

Present in vitro experiment was carried out to identify plant growth promoting rhizobial isolates for abiotic stress tolerance. Rhizospheric soils from normal, salt affected, drought affected were collected from different places of Telangana state. From all soil samples, twenty one rhizobial isolates were collected. Among the twenty one isolates, eleven isolates were showed different plant growth promoting properties. The results of different in vitro abiotic stress tolerance of isolates reveals that, only one isolate showed tolerance to temperature from 20ºC-50ºC (YR-21), three isolates were showed tolerance to temperature from 20ºC - 45ºC (RR-1, GR-5, GNR-8), one isolate was showed tolerance to temperature from 30ºC- 45ºC (MR-11), only one isolate were showed tolerance to temperature from 30ºC - 40ºC (ASR-17). Two isolates showed tolerance to water potential from - 0.05 Mpa to- 0.15 Mpa (RR-1, RR-3), three isolates were showed tolerance to water potential from - 0.05 Mpa to -0.30 Mpa (GNR-8, ASR-19, YR-21), only one isolate showed tolerance to water potential at - 0.05 Mpa (GNR-7).

1. Lupwayi N.Z, Clayton G.W., Hanson K.G., Rice W.A. and Biederbeck V.O. (2004) Canadian Journal of Plant Science.84,37–45.
2. Biswas J.C., Ladha J.K., Dazzo F.B., Yanni Y.G. and Rolfe B.G.(2000) Agronomy Journal; 92,880–886.
3. Chaintreuil C., Giraud E., Prin Y., Lorquin J., Ba A. and Gillis M. (2000) Appl Environmental Microbiology.66, 5437–5447.
4. Peoples M.B., Herridge D.F. and Ladha J.K. (1995) Plant Soil, 174, 3–28.
5. Harb A., Krishnan A., Ambavaram M.M.R. and Pereira A. (2010) Plant Physiology. 154 (3), 1254-1271.
6. Wei G.H., Yang X.Y., Zhang Z.X., Yang Y.Z. and Lindstrom K. (2008) Pedosphere, 18,102-112.
7. Kloepper J.W., Ryu C.M. and Zhang S.A. (2004) Phytopathology, 94, 1259-1266.
8. Yang L., Zhai L., Wang R., Zhao R., Zhang X., Chen C., Cao Y., Cao Y., Xu T., Ge Y., Zhao J and Cheng C. (2015) International Journal of Systematic and Evolutionary Microbiology, 65, 4533–4538.
9. Pikovskaya R.I. (1948) Microbiologia, 17, 362–370.
10. Prajapati M.C and Modi H.A. (2012) Life sciences Leaflets, 5, 71-75.
11. Saravanan V.S., Subramoniam S.R and Raj S.A. (2003) Brazilian Journal of Microbiology, 34, 121-125.
12. Gorden A.S and Weber R.P. (1951) Plant Physiology, 26, 192-195.
13. Ali S.Z., Sandhya V. and Rao L.V. (2013) Annals of Microbiology, 5, 1-10.
14. Jacobson C.B., Pasternak J.J. and Glick B.R. (1994) Canadian Journal of Microbiology, 40, 1019–25.
15. Schwyn B. and Neilands J.B. (1987) Analytical Biochemistry, 160, 47-56.
16. Skidmore A.M. and Dickinson C.H. (1976) Transactions and Journal of the British Ceramic Society, 66, 57-74.
17. Sandhya V., Ali S.Z., Grover M., Reddy G. and Bandi V. (2009) Journal of Plant Interactions.,6,1-14.
18. Deepika Chaudhary and Satyavir Singh Sindhu (2017) Legume Research, 40(1), 80-86.
19. Cunningham D. and Munns D. N. (1984) Soil Science, 48, 1273-1276.
20. Requena N., Perez-Solis E., Azcon-Aguilar C., Jeffries P. and Barea J.M. (2001) Applied and Environmental Microbiology, 67, 495-498.
21. Kulkarni S. and Nautiyal C. S. (2000) Current Microbiology, 40, 221-226.
22. Rodriguez H., Fraga R., Gonzalez T. and Bashan Y. (2006) Plant soil, 287, 15-21.
23. Karanja N. K. and Wood M. (1988) Plant Soil, 112, 15-22.
24. Singh R.P., Bauddh K., Sainger M., Singh J.S and Jaiwal P.K. (2011) Nitrogen use efficiency in higher plants under drought, high temperature, salinity and heavy metal contaminations, in Nitrogen Use Efficiency in Plants, eds Jain V., Kumar A.P., editors. New Delhi: New India Publishing Agency. 99 -123.
25. Rahmani H.A., Saleh rastin N., Khavazi K., Asgharzaden A., Fewer D., Kiani S. and Lindstrom K. (2009) World J. Microbiol. Biotechnol., 25, 591-600.
26. Kulkarni S. and Nautiyal C.S. (1999) Journal General and Applied Microbiology, 45, 213–220.
27. Cytryn E.J., Sangurdekar D.P., Streeter J.G., Franck W.L., Chang W.S., Stacey G., Emerich D.W., Joshi T., Xu, D. and Sadowsky M.J. (2007) Journal of Bacteriology, 189, 6751–6762.
28. Uma C., Sivagurunathan P. and Sangeetha D. (2013) International journal of current microbiology and applied sciences. 2(5), 228-232.
29. Abolhasani M., Lakzian A., Tajabadipour A. and Haghnia G. (2010) Australian Journal of Basic and Applied Sciences, 4(5), 882-886.
30. Rehman A. and Nautiyal C.S. (2002) Effect of drought on the growth and survival of the stress-tolerant bacterium Rhizobium spp. NBRI2505 sesbania and its drought-sensitive transposon Tn5 mutant, Springer-Verlag New York LLC., 45(5): 368-377.