IDENTIFICATION AND CHARACTERIZATION OF CAUSAL AGENT OF CERCOSPORA LEAF SPOT DISEASE OF OKRA

S.M. ZIA HASAN¹, MD. FIROSE HOSSAIN², ZANNATI FERDOUS ZAOTI³, MD. SAROAR JAHAN⁴, MD. FARUK HASAN⁵, MD. ASADUL ISLAM⁶, BISWANATH SIKDAR⁷*
¹Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
²Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
³Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
⁴Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
⁵Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
⁶Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
⁷Professor Joarder DNA & Chromosome Research Lab, Department of Genetic Engineering and Biotechnology, Faculty of Life and Earth Science, University of Rajshahi, Rajshahi-6205, Bangladesh
* Corresponding Author : sikdar2014@gmail.com

Received : 08-02-2018     Accepted : 01-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1015 - 1019
Int J Microbiol Res 10.3 (2018):1015-1019
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1015-1019

Keywords : Abelmoschus esculentus, Cercospora leaf spot, okra, fungi, Cercospora abelmoschi
Academic Editor : Dilruba Sarkar, Singh S. P., Md. Enamul Haque
Conflict of Interest : None declared
Acknowledgements/Funding : The authors wish to thanks, Ministry of Science and Technology - Government of the People's Republic of Bangladesh for providing financial support to carry the whole work. The authors also thanks to Professor Joarder DNA & Chromosome Research Lab, Department of Genetic Engineering and Biotechnology, University of Rajshahi for providing lab facilities.
Author Contribution : All authors are equally contributed

In august 2017, symptoms of leaf spot disease were found in okra (Abelmoschus esculentus L.) cultivated area of university of Rajshahi. Several morphological and biochemical tests were conducted for the identification and characterization of the causal agent of leaf spot disease of okra. The pathogen was isolated from infected leaf and morphological identification initially confirms the presence of the fungi of Cercospora spp. For the growth profiling, the fungi was grown in different media, temperature, pH, sugar concentrations, acid concentrations (Citric, Malic and Nicotinic Acid) and different carbohydrates (sucrose, fructose, maltose, glucose, lactose). The optimum growth of the fungi was observed at 25⁰C, pH 6 and 20% sugar concentration. The highest 43±0.3 mm radial colony growth was observed in Sucrose and Maltose with 0.457 gm and 0.435 gm dry weight. The fungi could not grow at 2% concentration of citric and malic acid. Cellulolytic activity was not observed for this strain. A BlastN search of 511bp obtained sequence was performed in GenBank database. And it revealed that, the strains had approximately 90% identity with several Cercospora spp. The obtained sequence deposited in GenBank (accession No. MG735450).

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