EFFECT OF 2,4D & EMSON IN VITRO REGENERATION IN SUGARCANE CULTIVAR, 2003V46

PRAVEEN KONA¹*, M. HEMANTH KUMAR², K.H.P. REDDY³, D.M. REDDY⁴, N.P. ESWAR REDDY⁵, P. LATHA⁶, M.S. BALAJI^7
1Department Crop Improvement Unit, ICAR- Directorate of Groundnut Research, Junagadh, 362001, Gujarat
²Department of Genetics and Plant Breeding, Agricultural Research Station, Perumallapalli, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
³Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
⁴Department of Genetics and Plant Breeding, S.V. Agricultural College, Tirupati, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
⁵Institute of Frontier Technologies, Regional Agricultural Research Station, Tirupati, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
⁶Institute of Frontier Technologies, Regional Agricultural Research Station, Tirupati, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
⁷Department of Genetics and Plant Breeding, Agricultural Research Station, Perumallapalli, 517 502, Acharya N. G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
* Corresponding Author : praveenkona61@gmail.com

Received : 23-03-2018     Accepted : 28-03-2018     Published : 30-03-2018
Volume : 10     Issue : 3       Pages : 1090 - 1093
Int J Microbiol Res 10.3 (2018):1090-1093
DOI : http://dx.doi.org/10.9735/0975-5276.10.3.1090-1093

Keywords : In vitro, Sugarcane, EMS, 2, 4D, Callus
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Agricultural Research Station, Perumallapalli, 517 502 staff for providing the lab facilities to carry out the research programme. Author thankful to Acharya N.G. Ranga Agricultural University, Guntur, 522034, Andhra Pradesh
Author Contribution : All are contributed equally

Studies were carried out to establish an efficient system for in vitro regeneration of sugarcane cultivar, 2003V46 using chemical mutagens; Ethyl methane sulphonate (EMS) and 2,4dichlorophenoxy acetic acid (2,4D). Young leaf rolls were used as explants for callus induction on MS medium containing different concentrations of EMS(0.6 µM l-1, 0.8 µM l-1 and 1.0 µM l-1) and 2,4D(4mg l-1, 5mg l-1 and 6 mg l-1). Among the different concentrations,2003V46 had taken minimum number of days (10.0) for callus induction with 2, 4D @ 4mg l-1,recordingmaximum mean callus size of 2.42 cm with high callus induction frequency (96.89%) and maximum mean number of explants inducing callus (2.86) in addition with superior response in shooting, rooting and hardening characters than EMS.2, 4D at higher concentration (6 mg l-1) had inhibitory effect on callusing, shooting and rooting and produced variation for leaf sheath hairiness, shape of bud and internode colour similar to that of the mutagen, EMS.

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