A preliminary investigation was performed about the presence of industrially important thermophilic bacteria in the hot springs and in its vicinity near Beramhapur, Orissa state, India. Out of 20 to 30 clones, the randomly picked up two clones of bacterium samples, one each from a soil and water, had been taken for the laboratory experiments. The cells isolated from soil and water samples stained gram negative, is rod shaped and non acid-fast organism, and positive for endospore staining. The temperature range for growth at a pH of 7.5 was between 37.5°C and 60°C with optimum growth at 50°C. Growth was inhibited by ampicillin, ciprofloxacin, cefadroxil and doxicycline, and cells showed positive protease, catalase and DNase activity, and lipase and oxidase activity was absent in them. The staining, morphological, biochemical, growth parameters, antibiotic studies proved that the bacteria are of gram negative and rod shaped nearer to the Escheritia coli cell type.

Polyene antibiotics are antifungal agents. They interact with cell membrane sterols and form pores in the membrane. All the cell constituents will leak through the pores and finally cell death occurs. Results of this paper have demonstrated that the specific phospholipids mutants of Saccharomyces cerevisiae which have been used to find out the the mechanism of action of antibiotic nystatin, by using the techniques of growth, viability and amino acid influx altered the sensitivity. The acquired resistance of phospholipids enriched cells as judged from (i) growth and viability could be dissociated (ii) the effect of antibiotics on the transport of amino acids was different from that of growth, viability and binding, which may due to the fact that membrane permeability changes could be dissociated. Further, the mutants, which accumulate PC (phosphatidyl choline) or PE (phosphatidyl ethanolamine) or PS (phospholipids serine) in the cell membrane, altered the sensitivity of antibiotic nystatin by non-specific binding of antibiotic to phospholipids. Further, these results would help for the pathogenesis control of Candida albicans, which is pathogenic yeast.

Twelve aryl acetic acid hydrazones were synthesized and tested first time for their antimicrobial activity. The structures of all these compounds were confirmed with the help of IR, 1HNMR, Mass spectra along with elemental analysis. Some of the target compounds show moderate activity against H37 RV strain of Mycobacterium tuberculosis at a concentration of 10μM.

A comparative bio-molecular assay was made on the two cyanobacterial isolates i.e. Anabaena cylindrica and Oscillatoria tenue to study the effect of different concentration of Diazinon, an organophosphorus insecticide. We have carried out these experiments to investigate the response of different concentration of Diazinon. Finally Maximum Allowable Concentration (MAC) of Diazinon on Cyanobacterial isolates was observed. Heterocystous forms of cyanobacteria are more sensitive to pesticide than that the non-heterocystus forms of cyanobacteria. Cyanobacterial isolates were treated with different concentrations of Diazinon i.e., 1ppm, 3ppm, 5ppm, 7ppm & 10ppm. The growth, macromolecular content of carbohydrate, protein, amino acid and nitrogen was observed. The experiments were carried out for 28 days, and it was observed that the higher concentration i.e., 9ppm and 10ppm of Diazinon are adversely affecting the growth & the macromolecular contents of cyanobacterial isolates. It is also found that at late exponential phase (even in untreated conditions) the experimental macromolecular content was high in the isolates.

The present investigation deals with the synthesis of some bromo-benzothiazolopyrazolines from substituted pyrazolines. The synthesized bromo benzothiazolopyrazolines were characterized by elemental analyses, IR, NMR and mass spectra. The fragmentation pattern of one of the compound has also been suggested.

Some new amides of 5-Sulphosalicylic acid were synthesized by the reaction of suitable amines with 5-sulphosalicylic acid in 2:1 ratio. The compounds are novel and first time screened for their biological evaluation along with general characterization with the help of elemental analysis, IR spectral analysis. These compounds showed higher to moderate biological activity against different pathogenic microbial strains.

Multi-parametric Quantitative structure activity relationship (QSAR) study has been developed for 110 training compounds and 50 test compounds structurally similar to 5-N-ACETYL-BETA-D-NEURAMINIC ACID as inhibitors for Clostridium tetani. Stepwise (multi-parametric) Linear Regression QSAR models for biological activity of half maximal inhibitory concentration (IC50) and log P for octanol/water (Log P) were created with 16 different descriptors. The predictive capability of the QSAR models were evaluated by r2 , q2 LMO(TestSet) , q2LOO(TestSet) , q2BOOT(TestSet). The comparison of various external validation reveals identical q2 LMO(TestSet) , q2 LOO(TestSet) and q2 BOOT(TestSet) for IC50 (0.98), and Log P(0.7) which demonstrates the high robustness and real predictive power of IC50 and Log P model. LMO-Leave many out, LOO-Leave one out, BOOT- bootstrapping