Nystatin Induced Changes in Growth, Viability and Amino acid Influx of Yeast Saccharomyces cerevisiae

Gopala Rao T.V.¹, Sudip Kumar Sen², Amruta Samal³, Soumya Satpathy^4
1School of Life Sciences, JNU-110067, India
²Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765022, Rayagada District, Orissa, India
³Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765022, Rayagada District, Orissa, India
⁴Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765022, Rayagada District, Orissa, India

Received : -     Accepted : -     Published : 15-06-2010
Volume : 2     Issue : 1       Pages : 8 - 17
Int J Chem Res 2.1 (2010):8-17
DOI : http://dx.doi.org/10.9735/0975-3699.2.1.8-17

Keywords : Saccharomyces cerevisiae, nystatin, polyene, growth, viability, phosphatidyle choline (PC) phospatidyle ethanolamine (PE), phospatidyl serine (PS)
Conflict of Interest : None declared

Polyene antibiotics are antifungal agents. They interact with cell membrane sterols and form pores in the membrane. All the cell constituents will leak through the pores and finally cell death occurs. Results of this paper have demonstrated that the specific phospholipids mutants of Saccharomyces cerevisiae which have been used to find out the the mechanism of action of antibiotic nystatin, by using the techniques of growth, viability and amino acid influx altered the sensitivity. The acquired resistance of phospholipids enriched cells as judged from (i) growth and viability could be dissociated (ii) the effect of antibiotics on the transport of amino acids was different from that of growth, viability and binding, which may due to the fact that membrane permeability changes could be dissociated. Further, the mutants, which accumulate PC (phosphatidyl choline) or PE (phosphatidyl ethanolamine) or PS (phospholipids serine) in the cell membrane, altered the sensitivity of antibiotic nystatin by non-specific binding of antibiotic to phospholipids. Further, these results would help for the pathogenesis control of Candida albicans, which is pathogenic yeast.

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