Characterization of hot water spring source isolated clones of bacteria and their industrial applicability

Gopala Rao T.V.¹*, Sudip Kumar Sen², Sudhir Kumar Mohapatra³, Soumya Satpathy^4
1Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022, Rayagada District, Orissa State, India
²Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022, Rayagada District, Orissa State, India
³Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022, Rayagada District, Orissa State, India
⁴Department of Biotechnology, Gandhi Institute of Engineering and Technology, Gunupur-765 022, Rayagada District, Orissa State, India
* Corresponding Author : gopalraotv@yahoomail.com

Received : -     Accepted : -     Published : 15-06-2010
Volume : 2     Issue : 1       Pages : 1 - 7
Int J Chem Res 2.1 (2010):1-7
DOI : http://dx.doi.org/10.9735/0975-3699.2.1.1-7

Keywords : thermophiles, bacteria, growth, biochemical assay, antibiotic assay
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are very much thankful to management of GIET, Gunupur-765022, for the facilities provided for this paper

A preliminary investigation was performed about the presence of industrially important thermophilic bacteria in the hot springs and in its vicinity near Beramhapur, Orissa state, India. Out of 20 to 30 clones, the randomly picked up two clones of bacterium samples, one each from a soil and water, had been taken for the laboratory experiments. The cells isolated from soil and water samples stained gram negative, is rod shaped and non acid-fast organism, and positive for endospore staining. The temperature range for growth at a pH of 7.5 was between 37.5°C and 60°C with optimum growth at 50°C. Growth was inhibited by ampicillin, ciprofloxacin, cefadroxil and doxicycline, and cells showed positive protease, catalase and DNase activity, and lipase and oxidase activity was absent in them. The staining, morphological, biochemical, growth parameters, antibiotic studies proved that the bacteria are of gram negative and rod shaped nearer to the Escheritia coli cell type.

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