Post-Harvest Laboratory were carried out to know the different sugar levels for preparation the pineapple candy and to investigate the physic-chemical changes during storage in order to assess acceptability as well as shelf life of the product. Out of three levels tested, the 75% total soluble solids treatment was found optimum for getting desired results. Ascorbic acid was totally lost during treatments and product preparation. There was a little progress in acidity and reducing sugar upto 60 days of storage period. After 60 days quality product decrease. Fruit processing with 75% total soluble solids observed best in phsico-chemicals during storage period.

The aim of this study was to screen the soil’s streptomyces sp. in Gorgan region in Iran and evaluation of antifungal activity of these bacteria against Trichophyton rubrum. A total of 25 strain of streptomyces isolates from different soil samples were identify and screened for their antifungal activity by well diffusion method. All isolates are being typed using biochemical and morphological characteristics. Ten isolates had antidermatophytic effects and two of them showed highest antagonistic activity to dermatophyte, these isolates identified by molecular method using PCR and 16s rRNA gene sequence analysis. Genetic analysis confirmed that antagonistic isolates close relationship with known streptomyces species with 99-100% sequence identity. Streptomyces sp. SXY37 showed highest antifungal activity against T. rubrum by well diffusion method with 23.6 mm zone of inhibition.

Phytochemicals screening in various plants had gained interest in formulation of herbal drugs. Many oxidative stress related diseases are as a result of accumulation of free radicals in the body. A lot of researches are going on worldwide directed towards finding natural antioxidants of plants origins. The aims of this study were to screen phytochemical constituents and evaluate in vitro antioxidant activity of leaves of different varieties of mulberry plant (Family Moraceae). The methanolic extract of leaves of different mulberry varieties namely S-13, S-54, S-146, BR-2, S-36, S-1 and AR-12 which belongs to Morus alba and Morus indica were subjected for the phytochemical screening. The preliminary screening reports the presence of phenolic compounds, flavonoids, terpenoids, alkaloids etc. in methanolic extracts. FRAP assay Ascorbic acid was used as standard for the both. On the basis of results obtained from the HPLC analysis, methanolic extracts of mulberry leaves samples of S-36, S-1, AR-12 varieties contain more amount of phenolics and flavonoids exhibiting high anti-oxidant activity. It also chelates iron and has reducing power. The in vitro FRAP assay clearly indicated that S-1 mulberry leaf extract showed maximum significant FRAP concentration at 800 and 1000µL/mL (0.408 ± 0.001 mmol FeSO4/100 g dried sample and 0.410 ± 0.001 mmol FeSO4/100 g dried sample respectively) a significant source of natural antioxidant, which might be helpful in preventing the progress of various oxidative stresses. However, the components responsible for the anti-oxidative activity are morin, querectin, resveratrol, scopoletin, 7-hydroxycoumarin. Furthermore, these mulberry varieties may be utilized in epidemiological studies.

PCR-RFLP analysis of exon 8 of Bone Morphogenetic Protein Receptor (BMPR IB) gene was performed on blood samples from 30 Garole sheep. Amplified PCR fragment of exon 8 of Bone Morphogenetic Protein Receptor IB (BMPR IB) gene produced 140 bp. The PCR-RFLP analysis using AvaII restriction enzyme showed only one type of restriction pattern yielding two fragments of sizes 110 and 30 bp in all the studied animals. The present results revealed the lack of polymorphism of Bone Morphogenetic Protein Receptor IB (BMPR IB) gene with respect to AvaII restriction enzyme and accordingly, the genotype and allelic frequencies for this fragment were 1.00 in the studied Garole sheep population. The presence of single genotype of Bone Morphogenetic Protein Receptor IB (BMPR IB) gene of Garole sheep in the present study may be due to small sample size and close herd. Hence, further study to be necessary on large number of samples for confirmation of the present findings.

Siderophores-pyoverdine is compound secreted under low iron stress that acts as specific ferric iron chelate agents and some actinides as thorium and uranium. Pseudomonas aeruginosa SHA 282 was identified by 16S rDNA sequencing. It was found to produce catechol and hydroxamate derivates of pyoverdine. The optimum conditions for maximum pyoverdine production was obtained on succinate medium at pH=7, where no iron concentration. The produced pyoverdine was purified by Amberlite IRA 402 resin. The structure of pyoverdine was confirmed by NMR and CA-MS analyses. The gel electrophoresis of pyoverdine appeared that it contained two protein bands at ~12.5 and 9.5 kDa. The gene responsible for pyoverdine production was identified. This compound was able to complex and chelat with 89.60% and 87.43% of thorium (IV) from (Th-U) concentrate and monazite, respectively.