ENHANCEMENT THE PRODUCTION OF SIDEROPHORES-PYOVERDINE BY Pseudomonas aeruginosa SHA 282 AND ITS CHELATION WITH THORIUM (IV)

HUSSIEN S.S.¹, DESOUKY O.A.², ABDEL-HALIEM M.E.F.³, EL-MOUGITH A.A.^4
1Nuclear Materials Authority, El-Maadi, Cairo, Egypt.
²Nuclear Materials Authority, El-Maadi, Cairo, Egypt.
³Department of Botany, Faculty of Science, Zagazig University, Egypt.
⁴Department of Botany, Faculty of Science, Zagazig University, Egypt.

Received : 09-03-2013     Accepted : 23-07-2013     Published : 12-08-2013
Volume : 1     Issue : 1       Pages : 17 - 23
World Res J Biotechnol 1.1 (2013):17-23

Siderophores-pyoverdine is compound secreted under low iron stress that acts as specific ferric iron chelate agents and some actinides as thorium and uranium. Pseudomonas aeruginosa SHA 282 was identified by 16S rDNA sequencing. It was found to produce catechol and hydroxamate derivates of pyoverdine. The optimum conditions for maximum pyoverdine production was obtained on succinate medium at pH=7, where no iron concentration. The produced pyoverdine was purified by Amberlite IRA 402 resin. The structure of pyoverdine was confirmed by NMR and CA-MS analyses. The gel electrophoresis of pyoverdine appeared that it contained two protein bands at ~12.5 and 9.5 kDa. The gene responsible for pyoverdine production was identified. This compound was able to complex and chelat with 89.60% and 87.43% of thorium (IV) from (Th-U) concentrate and monazite, respectively.