ISOLATION, MOLECULAR IDENTIFICATION AND EVALUATION OF ANTIFUNGAL ACTIVITY OF SOIL STREPTOMYCES AGAINST DERMATOPHYTES

PORDELI H.R.¹, HASHEMI S.J.², JAMSHIDIAN M.³, BAYAT M.^4
1Department of mycology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.
²Department of mycology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.
³Department of microbiology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.
⁴Department of mycology, Faculty of Specialized Veterinary Sciences, Islamic Azad University, Science and Research Branch, Tehran, Iran.

Received : 02-07-2012     Accepted : 11-07-2013     Published : 12-08-2013
Volume : 1     Issue : 1       Pages : 4 - 6
World Res J Biotechnol 1.1 (2013):4-6

The aim of this study was to screen the soil’s streptomyces sp. in Gorgan region in Iran and evaluation of antifungal activity of these bacteria against Trichophyton rubrum. A total of 25 strain of streptomyces isolates from different soil samples were identify and screened for their antifungal activity by well diffusion method. All isolates are being typed using biochemical and morphological characteristics. Ten isolates had antidermatophytic effects and two of them showed highest antagonistic activity to dermatophyte, these isolates identified by molecular method using PCR and 16s rRNA gene sequence analysis. Genetic analysis confirmed that antagonistic isolates close relationship with known streptomyces species with 99-100% sequence identity. Streptomyces sp. SXY37 showed highest antifungal activity against T. rubrum by well diffusion method with 23.6 mm zone of inhibition.