Organisms were isolated on their basis of survival in an iron-limited environment. The survivors of this treatment were largely actinomycetes. Of the viable cultures, most were found to produce siderophore like compounds. The most prolific producers as assessed by the Chromo Azuerol Sulphate assay were further characterised and found to belong to the genus Streptomyces. Attempts to taxonomically characterise these organisms illustrated the conserved nature of the 16S rRNA gene in this group of organisms. Physiological characterisation was undertaken and it was found that the resistance of these organisms to a range of antibiotics proved to be a useful discriminating factor .

Biological control of Aedes aegypti, the dengue virus vector, is needed to replace synthetic insecticides. This study demonstrated the insecticidal efficacy of water and ethanolic seed extracts of mintweed Hyptis suaveolens (MSE), yam bean Pachyrhizus erosus (YSE) and celery Apium graveolens (CSE) on controlling of Ae. aegypti larvae and adults. The cytotoxicity of the extracts was investigated by brine shrimp lethality assay (BSLA). Yam bean extracts were highly cytotoxic to brine shrimps. All seed ethanolic extracts were more toxic to mosquitoes than water extracts. The insecticidal efficacy of all extracts ranged as YSE/e > YSE/w > MSE/e > CSE/e > MSE/w > CSE/w. YSE/e was most toxic to Ae. aegypti 2nd instar larvae and adults with LC50 of 16.22 ± 0.20 μg/ml and 91.41 ± 0.49 μg/ml respectively and much higher than MSE/s and CSE/e. YSE in combinations produced strong synergistic effects to the other extracts. The insecticidal activities of MSE and CSE were mild. They could lessen the activity of YSE. This study demonstrates that the safe utilization of plant seed extracts, particularly of the very toxic yam bean seeds, as Ae. aegypti biological control agents are possible by either individuals or suitable combinations with other plant or seed extracts.

In Today’s world due to global warming and climate changes there is challenging situation in field of agriculture. Number of advanced methods and technologies are coming in agriculture to reduce cost and improve total productivity. Water is an important resource in agriculture. There is new concept of agriculture in controlled environment. To reduce other cost in agriculture i.e. soil testing. This paper concentrate on Measuring temperature, humidity, and controlling water supply depend on moisture content of soil.