IN VITRO TECHNOLOGIES OF ENDANGERED SPECIES Pterocarpus santalinus

S. VENNILA¹, S. KALA², C. CINTHIA FERNANDAZ³*, M.V. JAWAHAR VISHNU⁴, S. KIRUTHIKA^5
1Agricultural College and Research Institute, Tiruvanamalai, 606 753, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
²Senior Scientist (Forestry), ICAR-Indian Institute of Soil & Water Conservation, Research Centre, Kota, 324 002, Rajasthan, India
³UPASI Krishi Vigyan Kendra, Ooty, The Nilgiris, 643001, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁴Forest College and Research Institute (FC & RI), Mettupalayam, 641301, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁵Forest College and Research Institute (FC & RI), Mettupalayam, 641301, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
* Corresponding Author : cinthia.tnau@gmail.com

Received : 11-04-2023     Accepted : 28-05-2023     Published : 30-05-2023
Volume : 15     Issue : 5       Pages : 12325 - 12328
Int J Agr Sci 15.5 (2023):12325-12328

Keywords : Pterocarpus santalinus, in vitro propagation, Explant, Surface sterilization, Proliferation
Academic Editor : Dr Hemangi Mehta
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to UPASI Krishi Vigyan Kendra, Ooty, The Nilgiris, 643001, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
Author Contribution : All authors equally contributed

Studies on Pterocarpus santalinus were carried out in the tissue culture laboratory at Forest College and Research Institute (FC & RI), Mettupalayam, 641301 to standardize the in vitro propagation techniques. Sterilization of the shoot tip was done with 0.01% HgCl2 and NaOCl for 5 min for controlling the contaminant in nodal and intermodal segments. Among the explants tested for multiple shoot induction, intermodal segments proved good results. Among the two media viz., MS, White media, the species responded well to MS medium. The best treatment for obtaining multiple shoot induction was MS + 5.0 mgl-1 Kin + 2.0 mgl-1 BAP for shoot tip explants. For rooting of shoot tip cuttings 3 mgl-1 IBA was found to be the best treatment combination for maximum sprouting, rooting of cuttings, shoot length and root length

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