RNA-SEQUENCE AS AN AGRICULTURAL SCIENCE RESEARCH BOOSTER

M. BANIK¹*, A.K. MANDAL², NILANJAYA³, P. SHAH⁴, J. KUMAR^5
1Department of Plant Breeding and Genetics, Dr Rajendra Prasad Central Agricultural University, Pusa, 848125, Bihar, India
²Department of Agrometeorology, Dr Kalam Agricultural College, Arrabari, Kishanganj, 855107, Bihar Agricultural University, Sabour, 813210, Bihar, India
³Department of Plant Breeding and Genetics, Dr Rajendra Prasad Central Agricultural University, Pusa, 848125, Bihar, India
⁴Department of Genetics and Plant Breeding, SASRD, Nagaland University, Medziphema, 797106, Nagaland, India
⁵Department of Agriculture Engineering, Dr Kalam Agricultural College, Arrabari, Kishanganj, 855107, Bihar Agricultural University, Sabour, 813210, Bihar, India
* Corresponding Author : mahi.jit92@gmail.com

Received : 05-12-2022     Accepted : 28-12-2022     Published : 30-12-2022
Volume : 14     Issue : 12       Pages : 12006 - 12009
Int J Agr Sci 14.12 (2022):12006-12009

Keywords : RNA sequence method, High throughput sequencing, Expressed gene analysis
Academic Editor : Altino Choupina
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Plant Breeding and Genetics, Dr Rajendra Prasad Central Agricultural University, Pusa, 848125, Bihar, India. Authors are also thankful to Department of Agrometeorology, Dr Kalam Agricultural College, Arrabari, Kishanganj, 855107, Bihar Agricultural University, Sabour, 813210, Bihar, India and Department of Genetics and Plant Breeding, SASRD, Nagaland University, Medziphema, 797106, Nagaland, India
Author Contribution : : All authors equally contributed

RNA- sequence is a revolutionized next generation sequencing technology. This analysis does not demand gene probes rather provide an accurate measure of gene expression as compared to other commonly available techniques. The transcription and gene profile data can be store in database and could be accessed by researcher. Transcriptome is the collective information of all the gene readouts present in a cell including coding and non-coding RNA transcript. Genes that are differentially expressed during different developmental stages can be identified by aspect of transcriptomics. The fact that RNA sequencing does not entails gene probes rather provides precise measures of gene expression over a much wider range, which has demonstrated its credibility over other common technique. Thus, RNA sequencing gives very crucial genetic information along with alternative splicing and isoforms of genes

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