EFFICACY OF EGG YOLK AND SOYBEAN LECITHIN BASED SEMEN EXTENDERS FOR CRYOPRESERVATION OF KANKREJ BULL SEMEN

J.A. BHAVSAR¹*, B.M. RAJAN², B.N. SUTHAR³, P.T. SUTARIA^4
1Department of Animal Husbandry, Veterinary Officer, Thangadh, Surendranagar, 363530, Gujarat, India
²Department of Animal Husbandry, Veterinary Officer, Idar, Sabarkantha, 383430, Gujarat, India
³Department of Gynaecology and Obstetrics, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Kamdhenu University, Gandhinagar, 382010, Gujarat, India
⁴Department of Surgery and Radiology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Kamdhenu University, Gandhinagar, 382010, Gujarat, India
* Corresponding Author : jaybhavsar2050@gmail.com

Received : 04-11-2022     Accepted : 27-11-2022     Published : 30-11-2022
Volume : 14     Issue : 11       Pages : 11826 - 11828
Int J Agr Sci 14.11 (2022):11826-11828

Keywords : Kankrej bull, Cryopreserved semen, Pregnancy rate, Soybean lecithin
Academic Editor : Dr P.M. Chauhan, Ravjibhai Karshanbhai Chaudhari, Dr Amit Kumar
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Research Scientist, Livestock Research Station and College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Kamdhenu University, Gandhinagar, 382010, India
Author Contribution : All authors equally contributed

Total of 60 ejaculates from 6 Kankrej bulls were collected, split and processed using soybean lecithin based (AndroMed®) and egg yolk based (TFYG) extenders. The various semen parameters and pregnancy rate of cryopreserved semen were assessed. The mean values of individual motility, live sperm, abnormal sperm and acrosomal integrity were significantly (P < 0.05) higher in AndroMed® extended semen than the TFYG extended semen. The pregnancy rate was didn’t differ significantly in TFYG and AndroMed® extended semen. In conclusion, the animal protein-free extender based on soybean lecithin is a viable alternative to the traditional egg yolk-based extenders for cryopreservation of kankrej bull semen

1. Vishwanath R. and Shannon P. (2000) Animal Reproduction Science, 62, 23-53.
2. Mathur A.K., Srivastava R.S., Joshi A. and Kalra D.B. (1991) International Journal of Animal Science, 6, 79-81.
3. Celeghini E.C.C., Arruda R.P., Andrade A.F.C., Nascimento J., Raphael C.F. and Rodrigues P.H.M. (2008) Animal Reproduction Science 104, 119-131.
4. Watson P.F. and Martin I.C. (1976) Theriogenology, 6, 927-935.
5. Bousseau S., Brillard J.P., Marguant-Le Guienne B., Guerin B., Camus A. and Lechat M. (1998) Theriogenology, 50, 699-706.
6. Thibier M. and Guerin B. (2000) Animal Reproduction Science, 62, 233-251.
7. Stradaioli G., Noro T., Sylla L. and Monaci M. (2007) Theriogenology, 67, 1249-1255.
8. Thun R., Hurtado M. and Janett F. (2002) Theriogenology, 57, 1087-1094.
9. Aires V.A., Hinsch K.D., Mueller-Schloesser F., Bogner K., Mueller-Schloesser S. and Hinsch E. (2003) Theriogenology, 60, 269-279.
10. Vera-Munoz O., Amirat-Briand L., Diaz T., Vasquez L., Shcmidt E., Desherces S., Anton M., Bencharif D. and Tainturier D. (2009) Theriogenology, 71, 895-900.
11. Akhter S., Ansari M.S., Rakha B.A., Andrabi S.M.H., Iqbal S. and Ullah N. (2010) Theriogenology, 74, 951–955.
12. Gil J., Rodriguez-Irazoqui M., Lundeheim N., Soderquist L. and Roderiguez-Martinez H. (2003b) Theriogenology, 59, 1157–1170.
13. Papa F.O., Felicio G.B., Melo C.M., Alvarenga M.A., DeVita B., Avanzi B.R. and DellAqua J.A. (2010) Animal Reproduction Science, 121, 71-72.
14. Hancock J.L. (1951) Journal of Experimental Biology, 29, 445-453.
15. Roberts S.J., (2004) Veterinary Obstetrics and Genital Diseases, 2nd Indian Edn. CBS Publishers and Distributors, New Delhi, 712.
16. Kutty I.C., Joseph M. and Iyer C.P.N. (1996) Indian Journal of Animal Research, 17(2), 122-123.
17. Snedecor G.W. and Cochran W.G. (1994) Statistical methods. 8th Edn. Affiliated East-West Press, New Delhi, India.
18. Amirat L., Anton M., Tainturier D., Chatagnon G., Battut I. and Courtens J. (2005) Reproduction, 129, 535-543.
19. Braga M.Q., Franco R.V.R., Rodrigues L.F., Galeli G., Oliveira K.M., Reis F.A.C., Nishikawa M.F.A. and Moura E.P. (2007) Reproduction Fertility and Development, 19, 172-172.
20. Bumroongthai K., Banmairuroy P., Pisamai S., Singlor J. and Tummaruk P. (2012) KKU Veterinary Journal, 22(1), 51-61.
21. Janett F., Keo S., Bollwein H., Hassig M. and Thun R. (2005) Schweiz Arch Tierheilk, 147, 62.
22. Patil S., Honnappagol S.S., Arora V.K. and Tandle M.K. 2008) Indian Veterinary Journal, 85(2), 219-220.
23. Gil J., Lundeheim N., Soderquist L. and Rodriguez-Martinez H. (2003a) Theriogenology, 59, 1241-1255.
24. Karabinus D.S., Evenson D.P. and Kaproth M.T. (1991) Journal of Dairy Science, 74, 3836-48.
25. Muller-Schlosser F., Aires V., Hinsch E. and Hinsch K.D. (2001) 34th Conference on physiology and pathology of reproduction, Giessen.
26. Trotta M., Pattarino F. and Ignoni T. (2002) European Journal of Pharmaceutics and Biopharmaceutics, 53, 203-208.
27. Zhang S.S., Hu J.H., Li Q.W., Jiang Z.L. and Zhang X.Y. (2009) African Journal of Biotechnology, 8, 6476-6480.
28. Smith R.L., Berndston W.E., Unal, M.B. and Pickett B.W. (1979) Journal of Dairy Science, 62, 1297-303.