SCREENING OF SOYBEAN VARIETIES FOR EFFECTIVE SYMBIOSIS WITH INDIGENOUS RHIZOBIUM Spp

SHANTI¹, DIPTIMAYEE DASH²*
¹Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
²Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
* Corresponding Author : mdpt.dash@gmail.com

Received : 06-12-2021     Accepted : 27-12-2021     Published : 30-12-2021
Volume : 13     Issue : 12       Pages : 11000 - 11003
Int J Agr Sci 13.12 (2021):11000-11003

Keywords : Soybean, Indigenous rhizobia, Nodulation, Biomass and Leghaemoglobin
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Agricultural Microbiology, Indira Gandhi Krishi Vishwavidyalaya, Raipur, 492012, Chhattisgarh, India
Author Contribution : All authors equally contributed

The yield of soybean (Glycine max L. Merrill) can be alleviated by developing promising genotypes for efficient symbiosis. A field experiment was conducted at College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya, Raipur with the aim of screening of soybean germplasm for effective symbiotic association with indigenous Rhizobium sp. in order to identify potential genotypes for subsequent breeding work focusing on promiscuous effective nodulation. Eighteen soybean varieties grown in the Research field of College of Agriculture, Indira Gandhi Krishi Vishwavidyalaya were evaluated during kharif 2019-20 on the basis of measured traits like biomass accumulation; nodulation, leghaemoglobin content and symbiotic performance of soybean genotypes. The replicated soybean varieties were collected randomly at 50 % flowering stage and taken to the laboratory for the isolation of Rhizobium from nodules. The eighteen different soybean rhizobia isolate from 18 soybean varieties were taken as treatments. Colony growth in YEMA media of indigenous soybean rhizobia isolates and microbial activities in Soybean rhizosphere soils, it is seen that SRh-1(isolated from Var. NRCSL 1), SRh-03 (RSC 11-03), SRh-18 (RKS 18), SRh-137 (NRC 137) and SRh-136 (NRC 136) were promising indigenous Rhizobium isolates. The maximum nodule dry weight was recorded from the soybean cultivar NRCSL 1 (230mg plant-1) followed by RSC 11-03 (221mgplant-1). Similarly maximum shoot dry weight also observed in soybean cultivar NRCSL 1 (16.79g/plant) followed by RSC 11-03 (16.35g/plant). leghaemoglobin content was being significantly highest in the variety NRCSL 1 (1.77mg/g fresh weight of nodules). This study proposed that five soybean genotypes NRCSL1, RSC 11-03, RKS 18, NRC 137, NRC 136 as potential genotypes over other varieties for effective symbiosis with indigenous rhizobia

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