IN VITRO MICRO PROPAGATION OF SWEET ORANGE

S.S. DESHMUKH¹, M. GALPHADE², D.N. MUSKE³*
¹Biotechnology Center, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444104, India
²College of Agricultural, Biotechnology, Madadgaon, 414201, Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri, 413722, India
³College of Horticulture, Bidar, 585401, University of Horticultural Sciences, Bagalkote, 587104, Karnataka, India
* Corresponding Author : muskedeepa@gmail.com

Received : 02-12-2021     Accepted : 27-12-2021     Published : 30-12-2021
Volume : 13     Issue : 12       Pages : 10972 - 10974
Int J Agr Sci 13.12 (2021):10972-10974

Keywords : In-vitro multiplication, Citrus, Sweet orange, Micropopogation
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to College of Horticulture, Bidar, 585401, University of Horticultural Sciences, Bagalkote, 587104, Karnataka, India. Authors are also thankful to Biotechnology Center, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444104, India and College of Agricultural, Biotechnology, Madadgaon, 414201, Mahatma Phule Krishi Vidyapeeth (MPKV), Rahuri, 413722, India
Author Contribution : All authors equally contributed

Present investigation was conducted to standardize a protocol for in-vitro propagation of citrus spp. i.e. sweet orange for commercial purpose. The shoot tip explant was found better for callus induction of these plants than the nodal segment and epicotyls. Maximum callus formation (40.0% and 23.3% 22.2%) of shoot tip explants was obtained respectively in treatment MS basal media + 0.8mg/l Kinetin, 1.5mg/l NAA, and 2.5 mg/l 2, 4-D. Furthermore, the maximum number of shoots per explant was obtained through the callus in MS basal media + BA 1mg/l. Maximum rooting of shoots (1.11%) was noted sweet orange for the ½ MS media supplemented with 0.2 mg L-1 NAA plus 0.1 mg L-1 BA. Although the callus development and bud proliferation were recorded in all explants however, shoot and root formation did not occur. The potting media composing of soil, sand and FYM in the ratio of 1:1:1 by volume was better with maximum survival rate of hardened plants six weeks after transferring to the pots under greenhouse. In this way we can use this standardized protocol for regeneration of different rootstock for purity and uniformity purpose in seedling

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