CROSS LEGUME SPECIES/GENERA TRANSFERABILITY OF SSR MARKERS POLYMORPHIC TO PARENTS OF F2 MAPPING POPULATION IN BLACKGRAM (VIGNA MUNGO L. HEPPER)

M. KHANDAPPAGOL¹*, S. RANGAIAH^2
1Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
²Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
* Corresponding Author : mamatakhandappagol@gmail.com

Received : 03-12-2019     Accepted : 19-12-2019     Published : 30-12-2019
Volume : 11     Issue : 24       Pages : 9323 - 9325
Int J Agr Sci 11.24 (2019):9323-9325

Keywords : Vigna mungo, Blackgram, Transferability, Simple Sequence Repeat, Repeat Motifs, F2 population
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Genetics and Plant Breeding, College of Agriculture, University of Agricultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
Author Contribution : All authors equally contributed

Three hundred and ninety-five cross legume species/ genera SSR markers (65 from soyabean, 12 from medicago, 9 from chickpea, 105 from adzukibran, 70 from cowpea and 134 from mungbean) were used to identify those polymorphic to parents of F2 mapping population viz., TAU-1 and LBG-17 in blackgram, a genomic resource limited crop. Of these 395 SSR markers, 315 SSR markers (79.50%) amplified and 71 of them (22.62%) were polymorphic between the parents of F2 population. Among the 71 cross legume polymorphic SSR markers, those based on, di-nucleotide repeat motifs exhibited highest polymorphism (67.60 %), followed by tri- (18.30 %), complex-(9.85 %) and tetra- nucleotide repeat motifs (4.22 %). The identified polymorphism cross legume SSR markers is suggested for use in various applications in blackgram breeding research. The present study showed possibility of using cross legume species/ genera SSR markers in blackgram for genotyping the F2 mapping population for gene mapping and subsequently for marker assisted selection

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