OPTIMIZATION OF THE ULTRASONIC ASSISTED EXTRACTION PROCESS TO OBTAIN PHENOLIC COMPOUNDS FROM POMEGRANATE (Punica granatum) PEELS USING RESPONSE SURFACE METHODOLOGY

AHMAD FAIZAN1*, AMARDEEP KUMAR2
1Department of Post Harvest Engineering and Technology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202001, India
2Department of Post Harvest Engineering and Technology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202001, India
* Corresponding Author : f4faizahmad1989@gmail.com

Received : 23-11-2018     Accepted : 12-12-2018     Published : 15-12-2018
Volume : 10     Issue : 23       Pages : 7581 - 7585
Int J Agr Sci 10.23 (2018):7581-7585

Keywords : Ultrasonic extraction, Pomegranate peel, Phenolic, Flavonoid compounds
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Post Harvest Engineering and Technology, Faculty of Agricultural Sciences, Aligarh Muslim University, Aligarh, 202001, India
Author Contribution : All authors equally contributed

Cite - MLA : FAIZAN, AHMAD and KUMAR, AMARDEEP "OPTIMIZATION OF THE ULTRASONIC ASSISTED EXTRACTION PROCESS TO OBTAIN PHENOLIC COMPOUNDS FROM POMEGRANATE (Punica granatum) PEELS USING RESPONSE SURFACE METHODOLOGY." International Journal of Agriculture Sciences 10.23 (2018):7581-7585.

Cite - APA : FAIZAN, AHMAD, KUMAR, AMARDEEP (2018). OPTIMIZATION OF THE ULTRASONIC ASSISTED EXTRACTION PROCESS TO OBTAIN PHENOLIC COMPOUNDS FROM POMEGRANATE (Punica granatum) PEELS USING RESPONSE SURFACE METHODOLOGY. International Journal of Agriculture Sciences, 10 (23), 7581-7585.

Cite - Chicago : FAIZAN, AHMAD and AMARDEEP, KUMAR. "OPTIMIZATION OF THE ULTRASONIC ASSISTED EXTRACTION PROCESS TO OBTAIN PHENOLIC COMPOUNDS FROM POMEGRANATE (Punica granatum) PEELS USING RESPONSE SURFACE METHODOLOGY." International Journal of Agriculture Sciences 10, no. 23 (2018):7581-7585.

Copyright : © 2018, AHMAD FAIZAN and AMARDEEP KUMAR, Published by Bioinfo Publications. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution and reproduction in any medium, provided the original author and source are credited.

Abstract

Pomegranate peels are a potential source of bioactive compounds which are known for their antioxidant and antimicrobial activities. The present work aims to study, the optimization of ultrasonic assisted extraction (UAE) method to obtain phenolic compounds from pomegranate peels using ethanol as a solvent. It further aims to investigate the effect of three independent variables such as solvent–solid ratio, extraction temperature and extraction time on the response variables such as total yield (TY), total phenolic content (TPC) and total flavonoid content (TFC) using response surface methodology (RSM). The results of RSM revealed the optimized conditions of the solvent-solid ratio, extraction temperature and extraction time as 16.09, 30°C and 17.8 minutes respectively, with the maximum yield of 14.20%, TPC of 140.61 mg GAE/ml extract and TFC of 2.96 mg RE/ml extract. The results were further validated under the same conditions using UAE and then compared with the thermostatic water bath extraction method (TWE).

References

1. Zhou S.H., Fang Z.X., Lü Y., Chen J.C., Liu D.H. and Ye X.Q. (2009) Food Chemistry, 112(2), 394-399.
2. Chidambara Murthy K.N., Jayaprakasha G.K. and Singh R.P. (2002) J Agric Food Chem 50(17), 4791-4795.
3. Mertens-Talcott S.U., Jilma-Stohlawetz P., Rios J., Hingorani L. and Derendorf H. (2006) Journal of Agricultural and Food Chemistry, 54(23), 8956-8961.
4. Jaiswal V., DerMarderosian A. and Porter J.R. (2010) Food Chemistry, 118(1), 11-16.
5. Sreekumar S., Sithul H., Muraleedharan P., Azeez J.M. and Sreeharshan S. (2014) J BioMed Research International, 2014, 12.
6. Li Y., Guo C., Yang J., Wei J., Xu J. and Cheng S. (2006) Food Chemistry, 96, 254-260.
7. Sood A. and Gupta M. (2015) Food Science, 12, 100-106.
8. Sahin S. and Samli R. (2013) Ultrason Sonochem 20(1), 595-602.
9. Balasundram N., Sundram K. and Samman S. (2006) Food Chemistry, 99(1), 191-203.
10. Khan M.K., Abert-Vian M., Fabiano-Tixier A.S., Dangles O. and Chemat F. (2010) Food Chemistry, 119 (2), 851-858.
11. Banu K. S. and Cathrine L. (2015) International Journal of Advanced Research in Chemical Science, 2 (4), 25-32.
12. Singleton V.L. and Rossi J.A. (1965) American Journal of Enol. Vitic., 16(3), 144-158.
13. Saeed N., Khan M.R., Shabbir M. (2012) BMC Complementary and Alternative Medicine, 12(1), 221.
14. Nazni P. and Karunathara D. (2011) International Journal of Current Research, 3(8), 27-32.
15. Box G.E.P. and Wilson K.B. (1951) Journal of the Royal Statistical Society. Series B (Methodological), 13(1), 1-45.
16. Mohamed A. S., Chang Y. L. (2009) Food Chemistry, 108, 977–985.
17. Chemat F., Zille H. and Khan M.K. (2011) Ultrasonics Sonochemistry, 18(4), 813-835.