MITIGATION OF SALINITY STRESS EFFECT BY PLANT GROWTH REGULATORS AND NUTRIENTS IN BLACK GRAM UNDER INVITRO CONDITION

R. SIVAKUMAR¹*, S. JAYA PRIYA^2
1Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu
²Department of Crop Physiology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu
* Corresponding Author : sivatnau14@gmail.com

Received : 23-02-2018     Accepted : 26-02-2018     Published : 28-02-2018
Volume : 10     Issue : 4       Pages : 5199 - 5202
Int J Agr Sci 10.4 (2018):5199-5202
DOI : http://dx.doi.org/10.9735/0975-3710.10.4.5199-5202

Keywords : Jasmonic acid, salicylic acid, root length, vigour index, Na+/K+ ratio, blackgram
Conflict of Interest : None declared
Acknowledgements/Funding : The Authors would like to acknowledge the Professor and Head, Department of Crop Physiology, Tamil Nadu Agricultural University for providing Glass house, pots and labours for timely completion of Research work
Author Contribution : Sivakumar Rathinavelu – Design of experiment, data analysis and interpretation and drafting the article. Jaya Priya – Conduct the experiment, measurement of parameters and laboratory analysis.

An experiment was conducted to mitigate the impact of salinity stress on blackgram through seed soaking with plant growth regulators viz., jasmonic acid (50 µM), gibberellic acid (10 mM), benzyl amino purine (50 ppm),ascorbic acid (100 ppm),salicylic acid (100 ppm) and nutrients like KCl (1%) and CaCl2 (0.5%) under in-vitro condition. Salinity was imposed at125mM NaCl and seeds of TNAU (Blackgram) CO 6 were used in this study. The results revealed that salt stress caused detrimental effect on seed germination and seedling growth. Among the ameliorants, jasmonic acid showed significantly highest germination percentage of 86.50, vigour index of 771.58 and stress tolerance index of 39.38 per cent. Salinity reduced the root length up to 71.93 per cent compared to absolute control. The highest root length of 2.92 cm was registered by jasmonic acid followed by ascorbic acid (2.36 cm) which is on par with KCl (2.31 cm). However, salicylic acid recorded the least sodium/potassium ratio (0.320) followed by KCl (0.328), CaCl2 (0.329) and jasmonic acid (0.332). Jasmonic acid (5.55) and salicylic acid (5.85) showed their supremacy on increased catalase activity which protect the plant under saline condition.

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