ORGANOGENESIS AND IN-VITRO PLANTLETS PRODUCTION OF Aristolochia indica (Ishwarmul)

ANJU NEHRA¹, D.K. SHRIVASTAVA²*, PRIYANKA VERMA³, JAGDISH SINGH^4
1Department of Plant Breeding and Genetics, College of Agriculture, Indore, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M. P. India
²Department of Plant Breeding and Genetics, College of Agriculture, Indore, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M. P. India
³Department of Plant Breeding and Genetics, College of Agriculture, Indore, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M. P. India
⁴Department of Plant Breeding and Genetics, College of Agriculture, Indore, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, Gwalior, M. P. India
* Corresponding Author : shrivastavamedherbs@rediffmail.com

Received : 22-04-2017     Accepted : 27-04-2017     Published : 18-05-2017
Volume : 9     Issue : 23       Pages : 4259 - 4261
Int J Agr Sci 9.23 (2017):4259-4261

Keywords : Callusing, Induction, Explants
Academic Editor : Sudhanshu Jain
Conflict of Interest : None declared
Acknowledgements/Funding : Author are thankful to Department of Plant Breeding and Genetics, College of Agriculture, Indore, Rajmata Vijayaraje Scindia Krishi Vishwa Vidyalaya, M. P.
Author Contribution : All author equally contributed

The Aristolochia indica is a rare and endangered medicinal plant and also known as Snakeroot or Ishwarmul in Aurveda. It is famous in Indian System of Medicine as a antidote in bites of poisonous insects particularly the snake bite. It is also used in treatment of intestinal parasite, swelling, menstrual irregularities, white leprosy, dropsy, low appetite, ulcers and fever. For In-Vitro regeneration of Aristolochia indica, seven media were tested out of which five based on Murashige and Skoog’s medium (MS), one on Gamborg’s B5 and one on White’s media for their response on induction of callus from Stem segment, Leaf base and Cotyledons. Media based on MS responded well irrespective of explants used. Leaf base proved to be the best for callusing percent and fresh weight of callus in MS based media. The highest callusing efficiency was observed in MS medium with full strength of MS salts, 5mg/l 2,4-D+1.6mg/l BAP. Observation on shoot regeneration capacity suggested that MS medium contained MS salt +5mg/l 2,4-D+1.6 BAP has shown superior performance and was highly effective in inducing multiple shoots from callus. The MS medium with half strength of basic MS salts in combination with 1.2 mg/l BAP/ 0.6 mg/l IBA was also found to be the best for root regeneration. The Darkness supports the fast root regeneration in Aristolochia indica. The rooted plantlets were successfully transplanted in pots in poly house, after 25 days these plants with pots were transferred in to green house for further acclimatization under natural environments. The survival rate was 63%.

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