DIVERSITY ANALYSIS IN THE SELECTED F2 POPULATIONS OF WHEAT USING MOLECULAR MARKERS

PAWAN KUMAR¹, RAMESH KUMAR YADAVA²*, SANDEEP KUMAR³, PRITAM KUMAR^4
1Department of Genetics & Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004
²Department of Genetics & Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004
³Department of Genetics & Plant Breeding, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004
⁴Department of Molecular Biology and Biotechnology, Chaudhary Charan Singh Haryana Agricultural University, Hisar, Haryana 125004
* Corresponding Author : pk5gene@yahoo.com

Received : 05-02-2016     Accepted : 26-12-2016     Published : 28-12-2016
Volume : 8     Issue : 63       Pages : 3586 - 3590
Int J Agr Sci 8.63 (2016):3586-3590

Keywords : Genetic variability, SSR Marker, Spike morphology, F2 population
Academic Editor : Athanasios Tsafouros, VH Surve, Si Yong, Han Rong, Emilia L. Apostolova, Monalisha Biswal, Gholamian Asadollah, Jichao Sun
Conflict of Interest : None declared
Acknowledgements/Funding : None declared
Author Contribution : None declared

The present investigations were conducted to study the gene effects and molecular analysis for spike morphology in Triticum aestivum L.em Thell during two successive winter growing seasons (2008-2009 and 2009-2010). The phenotypic coefficients of variability (PCV) values were higher than GCV in the F2 population of five crosses for all the fifteen characters coupled with considerable differences between them indicating that the expression of these traits are more influenced or sensitive to the environmental conditions. Out of five crosses one diverse F2 populations were selected for molecular analysis. Fifty SSR primers were used for the present investigation, out of which only 42 primers showed amplification. Using a total of 42 SSR primers, 118 bands with the number of band per primer varying from 1 to 6 with an average 2.81 bands per primer. Average polymorphism across all the 100 F2 individuals was found to be 73.8%. Thus, a wide range of genetic variability was revealed from the similarity indices and dendrogram.