SINGLE STRAND CONFORMATION POLYMORPHISM (SSCP) OF BACILLUS SPECIES USING REC A CANDIDATE GENE APPROACH

LOPAMUDRA HALDAR¹*, D.N. GANDHI², S. DE^3
1Faculty of Science and Technology, ICFAI University, Tripura, Kamalghat, Tripura- 799210, India
²Dairy Microbiology Division, National Dairy Research Institute, Karnal- 132001, Haryana, India
³Animal Biotechnology Centre, National Dairy Research Institute, Karnal- 132001, Haryana, India
* Corresponding Author : mohor7@gmail.com

Received : 12-03-2016     Accepted : 19-03-2016     Published : 21-05-2016
Volume : 8     Issue : 17       Pages : 1300 - 1302
Int J Agr Sci 8.17 (2016):1300-1302

Keywords : Bacillus species, single strand conformational polymorphism, recA gene
Academic Editor : Dr Niladri Paul, Dr Santanu Banik
Conflict of Interest : None declared
Acknowledgements/Funding : The authors acknowledge the financial assistance and necessary supports provided by the Director NDRI, Karnal, Haryana, India to carry out the study. Sincere thanks to Dr. Rameshwar Singh, Project Director, ICAR-Directorate of Knowledge Management in Agriculture, New Delhi, India for his constant help and suggestions during the investigation at Dairy Microbiology Division, NDRI, Karnal, Haryana, India.
Author Contribution : None declared

The aim of the study was to isolate and characterize Bacillus species, which could be used as probiotic candidates. Initially, Bacillus isolates were collected from milk, cream, fresh tomato and soil. Based on morphological and biochemical tests, 37 Bacillus isolates including 3 standard cultures were subjected to molecular characterization using polymerase chain reaction (PCR) single strand conformation polymorphism (SSCP) technique with partial recA gene. The SSCP analysis of 263 bp partial recA amplicons of Bacillus isolates yielded complex SSCP patterns on 8% polyacrylamide gel. On the basis of SSCP band pattern of partial recA PCR products, all the selected isolates were classified into twelve different groups. The SSCP analysis of partial recA PCR products was found to be a powerful tool for illustrating polymorphism or heterogeneity within Bacillus species.