HIGH YIELD PURIFICATION OF VIABLE WHITE SPOT VIRUS PARTICLES FROM INFECTED PENAEUS MONODON

UBAID QAYOOM¹*
¹Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Versova, Andheri West, Mumbai, 400061, Maharashtra, India
* Corresponding Author : uqd2022@gmail.com

Received : 10-10-2022     Accepted : 28-10-2022     Published : 30-10-2022
Volume : 14     Issue : 10       Pages : 11791 - 11793
Int J Agr Sci 14.10 (2022):11791-11793

Keywords : Tiger shrimp, Aquaculture, Virus, Disease
Academic Editor : Dr Gulshan Kumar
Conflict of Interest : None declared
Acknowledgements/Funding : Author is thankful to Fish Genetics and Biotechnology Division, ICAR-Central Institute of Fisheries Education, Versova, Andheri West, Mumbai, 400061, Maharashtra, India
Author Contribution : Sole author

White spot syndrome caused by white spot syndrome virus (WSSV) is one of the most threatening diseases of shrimp culture industry. Globally, research is being carried out aiming at a better understanding on biology and pathology of WSSV and sub-sequential proper treatment and prevention. An efficient isolation and purification of viral particles is essential for these studies. This study reports a yield purification of viable and virulent WSSV virions from infected Peneaus monodon. The obtained yield was 8.64×1010 WSSV virions per 5 g infected tissue. Additionally, the yield dynamics of the complete process was studied for understanding of copy number variations. The present methodology and the yield-dynamics study of the process will lead to more improvements to WSSV purification in terms of simplicity and efficiency in the future. Moreover, the study will help in identification of the structural proteins of WSSV and shrimp bioassays to reveal the mechanisms of WSSV–host interaction

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