DETECTION OF VIRULENCE GENE OF STREPTOCOCCUS SPP. ISOLATED FROM BOVINE MASTITIS

A.K. THAKOR¹*, B.R. PANDOR², S.S. PATEL³, A.C. PATEL⁴, S.K. MOHAPATRA⁵, K.K. SHARMA^6
1Department of Veterinary Microbiology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
²Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
³Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
⁴Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
⁵Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
⁶Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, India
* Corresponding Author : akashthakore@gmail.com

Received : 10-10-2022     Accepted : 28-10-2022     Published : 30-10-2022
Volume : 14     Issue : 10       Pages : 11776 - 11779
Int J Agr Sci 14.10 (2022):11776-11779

Keywords : Mastitis, Gene amplification, Virulence
Academic Editor : Dr Gowher Gull Sheikh, Zalavadiya Denish, Dr Dhaval Chaudhary, Dr Prashant Shrivastava, Dr Gangavarapu Subrahmanyam
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Veterinary Microbiology; Department of Animal Biotechnology, College of Veterinary science & Animal Husbandry, Sardarkrushinagar, 385506, Dantiwada, Kamdhenu University, Gandhinagar, 382010, Gujarat, India
Author Contribution : All authors equally contributed

Mastitis occurs worldwide among dairy animals, and it has been described to have an extreme economic impact, despite the control strategies. This study aimed to investigate Streptococcus spp. Virulence gene found in clinical and subclinical mastitis milk samples from cows and buffaloes. The study was carried out on 285 milk samples collected from various area near to Banaskantha, Gujarat. Out of 285 milk samples (18 from subclinical and 95 from clinical cases) screened for Streptococcus spp. 17 isolates were obtained giving an overall incidence of 5.96 per cent. The incidence of Streptococcus spp. from subclinical mastitis was 2.63 per cent (5/190) and from clinical mastitis was 12.63 per cent (12/95). Species wise incidence was 8.10 per cent (6/74) in buffaloes and 5.21 per cent (11/211) in cows. All these 17 isolates were further confirmed as Streptococcus spp. using genus specific PCR All the 17 isolates were further confirmed using species-based PCR as Streptococcus agalactiae, Streptococcus dysgalactiae and Streptococcus uberis specific primers based Abstract gene amplification. In species specific PCR, Streptococcus agalactiae (n=4) (23.52%) produced 405 bp size amplicon, Streptococcus dysgalactiae (n= 6) (35.29%) produced 281 bp size amplicon whereas Streptococcus uberis (n=7) (41.17%) yielded 94 bp size amplicon. All the 17 isolates were further investigated for presence of virulence gene and out of 17 isolates of Streptococcus spp. 6 (35.29%) isolates were found positive for ScpB gene in which amplicon size of 255 bp while detected were other virulence gene bca, Rib, cylE found negative

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