MOLECULAR DIVERSITY ANALYSIS OF SOME AROMATIC RICE (ORYZA SATIVA L.) GENOTYPES USING SSR MARKERS

W. RANI¹, S.K. NOREN²*, M. RAI³, W. TYAGI⁴, V.K. KHANNA^5
1College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
²College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
³College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
⁴College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
⁵College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
* Corresponding Author : norensingh27@gmail.com

Received : 30-06-2019     Accepted : 12-07-2019     Published : 15-07-2019
Volume : 11     Issue : 13       Pages : 8713 - 8718
Int J Agr Sci 11.13 (2019):8713-8718

Keywords : Aromatic Rice, SSR, Genetic Diversity, Cluster Analysis, PIC
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to College of Post Graduate Studies in Agricultural Sciences, Central Agricultural University, (Imphal), Umiam, 793103, Meghalaya, India
Author Contribution : All authors equally contributed

Rice is one of the world’s most important cereal crops. It belongs to grass family Poaceae (2n = 24). There are 25 species of genus Oryza, and only two species, namely Oryza sativa and Oryza glaberrima are cultivated. Aromatic rice constitutes a small but an important sub-group of rice. The genetic diversity study was done using 24 SSR markers out of which 11 were polymorphic. The dendrogram generated by using Darwin 6.0.15 software identified three major clusters (I, II, and III). Cluster I is the largest cluster with 15 genotypes, Cluster II is the second largest cluster with 13 genotypes and Cluster III consisted of 4 genotypes. Among the polymorphic markers, number of alleles ranged from 2 (RM11, RM25 and RM 552), 3 (RM125), 4 (RM44, RM316, RM215 and RM271) and 6 (RM447). Highest PIC value was recorded for RM447 (0.750) and lowest for RM125 (0.236). Heterozygosity (He) value ranged from 0.127 (RM484) to 0.782 (RM447). Maximum diversity was observed between IC-137401 and IC-342368 (0.98), followed by IC-342368 and IC-326284 (0.97). The genetic diversity studies conducted will help in further crop improvement programmes in identification and germplasm preservation.

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