IDENTIFICATION OF A RFLP BASED MARKER FOR DOOM PIG OF ASSAM

K.B. KALITA¹*, S. LASKAR², L. BURAGOHAIN³, P. KAUSHIK^4
1College of Veterinary Science, Khanapara, Guwahati, 781022, Assam agriculture university, Jorhat, 785013, Assam, India
²College of Veterinary Science, Khanapara, Guwahati, 781022, Assam agriculture university, Jorhat, 785013, Assam, India
³College of Veterinary Science, Khanapara, Guwahati, 781022, Assam agriculture university, Jorhat, 785013, Assam, India
⁴College of Veterinary Science, Khanapara, Guwahati, 781022, Assam agriculture university, Jorhat, 785013, Assam, India
* Corresponding Author : kabitakalita2011@gmail.com

Received : 15-05-2019     Accepted : 12-06-2019     Published : 15-06-2019
Volume : 11     Issue : 11       Pages : 8563 - 8565
Int J Agr Sci 11.11 (2019):8563-8565

Keywords : Doom pig, RFLP, Cytochrome b gene
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to DBT and State Biotech Hub for the financial support during the research work. Authors are also thankful to College of Veterinary Science, Khanapara, Guwahati, 781022, Assam agriculture university, Jorhat, 785013, Assam, India
Author Contribution : All authors equally contributed

The study was carried out for analysing polymorphism of Cytochrome b (cyt b) gene of Doom pig and certain other exotic breed of pig found in Assam, and development of a RFLP based marker targeting the cytochrome b (cyt b) gene of Doom pig. An attempt was made by evaluating a partial cytochrome b (cyt b) gene sequence of 474 bp to differentiate Doom pig from other exotic animals in Assam. The PCR product was digested using the enzyme Stu I. Although the 474 bp fragment of the cyt b gene under study could not be used as an effective RFLP marker to identify the Doom pigs of Assam but two potential SNPs were detected at position 14944 and 15165 upon sequence analysis which could be used to differentiate Doom pigs from exotic breeds, viz. Hampshire and Yorkshire. The pair wise distance of partial cytochrome b gene at nucleotide levels revealed 99.3% and 99% identity of Doom pig with Hampshire and Yorkshire pigs, respectively. The study indicates that Doom pigs are closely related to Hampshire breed of pigs as revealed upon phylogenetic analysis.

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