DEVELOPMENT OF ACORUS CALAMUS L. NANOEMULSION AND THEIR INSECTICIDAL ACTIVITY AGAINST PULSE BEETLE (CALLOSOBRUCHUS MACULATUS F.)

V. DHIVYA¹, S.J. NELSON²*, K.S. SUBRAMANIAN³, Y.S.J.T. EDWARD⁴, K. RAJAMANI⁵, V.P. SANTHANAKRISHNAN⁶, S. SITHANANTHAM^7
1Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
²Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
³Department of Nano science and Technology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁴Department of Agricultural Entomology, Agricultural College and Research Institute, Thiruvannamalai, 606753, Tamil Nadu Agricultural University, Coimbatore, 641003
⁵Department of Medicinal and Aromatic Crops, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁶Department of Plant Biotechnology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
⁷Director Projects, Sun Agro Biotech Research centre, Chennai, 600116, Tamil Nadu, India
* Corresponding Author : sjn652003@gmail.com

Received : 25-04-2019     Accepted : 12-05-2019     Published : 15-05-2019
Volume : 11     Issue : 9       Pages : 8387 - 8390
Int J Agr Sci 11.9 (2019):8387-8390

Keywords : Acorus Calamus, Nanoemulsion, Callosobruchus Maculatus, Ultrasonication and Emulsion
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to Department of Agricultural Entomology, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India for financial support
Author Contribution : All authors equally contributed

In this study sweet flag (Acorus calamus L.) O/W nano emulsion was developed by ultra-sonication method. The macro- scale emulsion developed by adding 6 % A. calamus oil, a blend of tween 80 and span 80 with Hydrophile-Lipophile Balance (HLB) value-10.0 and cyclohexane. The developed macro- scale emulsion was subjected to ultra-sonication for 30 minutes at a high frequency of 20 kHz. The characterization in a Scanning electron microscope (SEM) showed that droplet diameter is in nano range and spherical in appearance. The mean droplet size, polydispersity index, and zeta potential were 68.2 nm, 0.21 and 64.3 mV respectively. The lethal concentration (LC50) value on C. maculatus for nanoemulsion was 0.40 % while it was 0.74 % for the micro- scale emulsion. Nanoemulsion of A. calamus has shown enhanced insecticidal activity at a lower concentration.

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