STERILITY MODULATION IN TGMS SYSTEM USING PLANT GROWTH REGULATORS FOR HYBRID RICE PRODUCTION

R. GAYATHRI¹, S. ROY²*, M.M. VIJI³, R. BEENA⁴, R.V. MANJU^5
1Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
²Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
³Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
⁴Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
⁵Department of Plant Physiology, College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
* Corresponding Author : roykau@gmail.com

Received : 01-12-2018     Accepted : 27-12-2018     Published : 30-12-2018
Volume : 10     Issue : 24       Pages : 7671 - 7674
Int J Agr Sci 10.24 (2018):7671-7674

Keywords : Pollen development, Male sterility, Pollen sterility, TGMS rice, Plant growth regulator
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to the Associate Director of Research, RARS, Ambalavayal for permitting to conduct the experiment in the high altitude research station. Authors are also thankful to Kerala Biotechnology commission for providing research grant. Authors are grateful to College of Agriculture, Vellayani, Thiruvananthapuram, 695522, Kerala Agricultural University, Thrissur, 680656, Kerala, India
Author Contribution : All authors equally contributed

Thermo–sensitive genic male sterility (TGMS) is a system in which temperature controls the sterility/fertility expression. TGMS genes are conditioned by temperature variations during critical thermosensitive stage. TGMS plants become male sterile when temperature is above critical sterility temperature (CST) and it remains fertile if the temperature is below the CST. It can overcome the difficulties in three line system of hybrid rice development and can successfully be utilized in tropical countries where significant variations in temperature exist between season and between altitudes. Frequent fluctuations in temperature can affect pure hybrid seed set in TGMS rice since complete male sterility is a prerequisite for hybridisation programmes. Phytohormones play a key role in attaining functional spikelet development. They are also involved in the thermo signalling pathway and control the male reproductive development in rice. Hence this study aims at identifying effective plant growth regulator in modulating pollen development and controls the expression of tms gene. Three plant growth regulators (PGR) namely ethrel, salicylic acid and maleic hydrazide (MH) were applied as foliar spray at two stages viz., panicle initiation and two weeks after panicle initiation. The results have shown that MH, ethrel and salicylic acid at different concentrations were effective in influencing anther development and induced pollen sterility. The study suggests that spraying of MH (8.92 mol m-3) two times; at the time of panicle initiation and fifteen days after panicle initiation were more efficient in inducing pollen sterility in TGMS rice and can be used for commercial hybrid rice seed development.

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