LIFE CYCLE AND HISTOPATHOLOGICAL STUDIES OF ROOT KNOT NEMATODE, Meloidogyne incognita IN SPONGE GOURD, Luffa cylindrica IN WESTERN UTTAR PRADESH, INDIA

RAJENDRA SINGH¹*, MONIKA SINGH^2
1Plant Nematology Research Lab, P.G. Department of Zoology, Bareilly Collage Campus, MJP Rohilkhand University, Bareilly, 243006, Uttar Pradesh, India
²Plant Nematology Research Lab, P.G. Department of Zoology, Bareilly Collage Campus, MJP Rohilkhand University, Bareilly, 243006, Uttar Pradesh, India
* Corresponding Author : singh.rajendra007@gmail.com

Received : 20-10-2018     Accepted : 12-11-2018     Published : 15-11-2018
Volume : 10     Issue : 21       Pages : 7457 - 7460
Int J Agr Sci 10.21 (2018):7457-7460

Keywords : Life cycle, Luffa cylindrica, Meloidogyne incognita, root knot nematode, sponge gourd
Academic Editor : Dr B. S. Vyakarnahal
Conflict of Interest : None declared
Acknowledgements/Funding : Authors are thankful to University Grant Commission (UGC) of Government of India for financial support for research. Authors are also thankful to P.G. Department of Zoology, Bareilly Collage Campus, MJP Rohilkhand University, Bareilly, 243006, Uttar Pradesh, India. These findings are the part of a major research project (F. No. 41-498/2012 (SR) funded by University Grant Commission (UGC) of Government of India. The authors are grateful to chairman and sanctioning body of UGC
Author Contribution : All authors equally contributed

Plant parasitic nematodes generally have a wide host range. Root knot nematode, Meloidogyne sps. is one of the most notorious parasites of plants which inhabits more than 3000 plants. This parasite considerably hampers the yield losses in different crops. In the present study an experiment has been setup to study the life cycle of commonly occurring species Meloidogyne incognita in sponge gourd, Luffa cylindrica L. as host plant. The life cycle and development of Meloidogyne incognita, was studied in the seedlings of sponge gourd grown in steam sterilized sandy loam soil in 15 cm diameter earthen pots placed in a screen house. Two weeks old sponge gourd seedlings were inoculated with about 1,000 freshly hatched second stage juveniles (J2) of M. incognita. Subsequently on a daily basis, two-three seedlings were randomly uprooted to examine the nematode development. Observations reveal that root-knot nematode, Meloidogyne incognita completes its life cycle from second stage juvenile (J2) to J2 of next generation within 28-30 days at atmospheric temperature ranging between 23°C- 40°C in roots as well as in soil. After searching the suitable feeding young root J2 penetrate the roots perpendicular to vascular system. Active feeding by penetrated J2 lead in second and third moulting as a result sedentary third (J3) and fourth stage juveniles (J4) were produced. J4 very soon changes into saucer shaped female followed by immature pear-shaped female. Immature female gets changed into mature female after 20-22 days of penetration. In next 6-7 days mature females were observed to deposit eggs in gelatinous matrix in their posterior region. After completion the first moulting inside the eggs, second stage juveniles (J2) were found to hatch from egg masses in successive 3-4 days.

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