STANDARDIZATION OF PROTOCOL FOR THE IN VITRO MICROPROPAGATION AND MASS PRODUCTION OF AN ENDANGERED MEDICINAL PLANT, Ophiorrhiza mungos L.

B.V. NAGESHA¹*, UGRAIAH CHOWDARY², B.L. MANJUNATH³, NATARAJ KARABA⁴, G.RAVIKANTH⁵, R. UMA SHAANKER^6
1Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
²Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
³Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
⁴Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
⁵Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
⁶Department of Crop Physiology, University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India School of Ecology and Conservation, University of Agricultural Sciences, GKVK, Bengaluru, 560065, India
* Corresponding Author : nageshgowdabgh06@gmail.com

Received : 20-09-2018     Accepted : 26-09-2018     Published : 30-09-2018
Volume : 10     Issue : 18       Pages : 7259 - 7262
Int J Agr Sci 10.18 (2018):7259-7262

Keywords : Ophiorrhiza mungos, Camptothecin, Topotecan, In vitro propagation, DNA topoisomerase I
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to University of Agricultural Sciences, GKVK, Bengaluru, 560 065, India
Author Contribution : All author are equally contributed

Camptothecin (CPT), an indole alkaloid compound. Topotecan and irinotecan, are CPT derivatives approved by Food and Drug Administration (FDA) were effectively used for treating various cancer. We have proved that biotechnological potential of O. mungos for mass multiplication from in vitro grown plants an efficient protocol for the regeneration of endangered O. mungos plants using nodal explants were developed. Initially explants obtained from seed germination of O. mungos. Sterilized seeds of O. mungos were transferred aseptically into MS medium and seed were germinated after 21 days. The nodal explants were inoculated on Murashige and Skoog (MS) medium fortified with different concentrations of plant growth regulator N6-benzyladenine (BA) and Thidiazuron (TDZ). The results revealed that the maximum number of shoots (23.06) from nodal explants obtained in MS medium fortified with 2mg/l BA within eight weeks. The maximum shoot elongation (3.76cm) obtained in MS medium fortified with 1.0mg/l Gibberellic acid (GA3) within four weeks. The regenerated shoots rooted well in the MS medium contain 1mg/l Indole-3-butyric acid (IBA). The rooted plantlets were successfully acclimatized with 100% survival rate in a growth chamber at 25°C, 60 % relative humidity, with 16-hrs photoperiod. The present findings indicate that O. mungos respond favourably for in vitro propagation and these in vitro regenerated flowering plants of O. mungos will be used for over expression of key genes involved in regulating terpenoid indole alkaloid (CPT) biosynthesis.

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