CHARACTERIZATION OF SUGARCANE MOSAIC VIRUS (SCMV) CAUSING MOSAIC DISEASE IN SUGARCANE

N. RAJA KUMAR¹*, K. VIJAY KRISHNA KUMAR^2
1Regional Agricultural Research Station, Anakapalle, 531001, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
²Regional Agricultural Research Station, Anakapalle, 531001, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India
* Corresponding Author : rajkumar1016@gmail.com

Received : 03-08-2018     Accepted : 26-09-2018     Published : 30-09-2018
Volume : 10     Issue : 18       Pages : 7251 - 7254
Int J Agr Sci 10.18 (2018):7251-7254

Keywords : Sugarcane, Mosaic, Molecular Characterization, ELISA electron microscopy
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Regional Agricultural Research Station, Anakapalle, 531001, Acharya N. G. Ranga Agricultural University, Lam, 522034, Andhra Pradesh, India. The authors are also grateful to Dr V K Barnawal, Advanced Centre for Plant Virology, Division of Plant Pathology, Indian Agricultural Research Institute, Pusa, New Delhi, 110012, India for Scanning Electron Microscopy analysis
Author Contribution : All author equally contributed

Sugarcane mosaic caused by Sugarcane Mosaic Virus (ScMV) is a serious problem in India’s sugarcane production. ScMV was first reported in India from Pusa during 1921 in sugarcane variety D-99 and now it has been reported in every sugarcane growing areas across India due to its perpetuation through vegetative cuttings and regarded it as a potential threat to sugarcane industry. Our present study was focused on characterization of ScMV and use of PGPR strains to manage the disease. A survey was undertaken in sugarcane grown areas of Andhra Pradesh, India and were found the sugarcane aphid (Melanophis sacchari) and corn leaf aphid (Rhopalosiphum maidis) as potential vectors for ScMV. Vector transmission was confirmed using DAC-ELISA. Further, the ScMV was detected in diseased leaves through DAC-ELISA and RT-PCR during our survey. Scanning Electron Microscopy (SEM) was also used to detect ScMV from diseased leaf samples. The results showed that all the leaf samples collected were shown positive reaction to the presence of ScMV in RT-PCR with a band at around 0.98 kbp. Further, asymptomatic leaves were also have shown positive reaction with RT-PCR for the presence of ScMV. Whereas, SEM studies showed the presence of poty-virus filamentous particles related to ScMV.

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