MOLECULAR CHARACTERISATION OF CASHEW (Anacardium occidentale L.) VARIETIES GROWN IN MAHARASHTRA USING ISSR MARKER

S.R. KAMBALE¹, ANIL BUKYA²*
¹Plant Biotechnology Centre, Dr Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli Ratnagiri, Maharashtra 415 712, India
²College of Food Technology, Saralgaon, Murbad, Thane, 421401, Dr Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli Ratnagiri, Maharashtra 415 712, India
* Corresponding Author : anilrathod23@gmail.com

Received : 18-08-2018     Accepted : 26-08-2018     Published : 15-09-2018
Volume : 10     Issue : 17       Pages : 7025 - 7028
Int J Agr Sci 10.17 (2018):7025-7028

Keywords : Cashew, Molecular Marker, ISSR, Genetic variation
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Plant Biotechnology Centre, Dr Balasaheb Sawant Konkan Krishi Vidyapeeth, Dapoli, 415712, Maharashtra, India
Author Contribution : All author equally contributed

The present study was carried out to standardize a DNA isolation protocol for cashew and to estimate genetic diversity among 9 cashew varieties developed by Dr. B. S. K. K. V. Dapoli, Maharashtra through the characterization by using 27 inter-simple sequence repeat (ISSR) markers. The DNA was extracted from the green leaf samples collected from 9 Cashew varieties. A protocol using 0.095 g/ml glucose, 0.025 g/ml polyvinylpyrrolidone, 0.0045 g/ml sodium bisulphite, 0.0055 g/ml sodium dodecyl sulphate, and 50 μl/ml sarcosine produced good quality DNA. A total of 1152 bands were scored out of which 882 were polymorphic, which showed 73.52% polymorphism. The size of amplified fragment ranged from 300 bp to 2000 bp. The primer UBC-843 recorded minimum PIC (Polymorphic information content) value 0.25, whereas primer UBC-876 gave maximum PIC value 0.91. The average PIC value was 0.70 among the all 9 varieties. The Jaccards’s Similarity Co-efficient ranged from 0.381 (between varieties Vengurla-2 and Vengurla-8) to 0.649 (between varieties Vengurla-4 and Vengurla-5). The maximum similarity coefficient indicates that all type varieties have narrow genetic base.

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