ISOLATION AND SCREENING OF PECTINOLYTIC BACTERIA (Bacillus megaterium) FROM THE MANGO FRUIT YARDS

K. SRIDEVI¹*, G. VENKATESH², M. SUMANTH³, K. VIJAYALAKSHMI^4
1SGS Arts College, Sri Venkateswara University, Tirupati, 517502, Andhra Pradesh, India
²Contractual Teacher, College of Horticulture, Anantharajupeta, Dr Y.S.R. Horticultural University, Venkataramannagudem, 534101, Andhra Pradesh, India
³Shanmugha Arts, Science, Technology & Research Academy, Thirumalaisamudram, Thanjavur, 613401, Tamil Nadu, India
⁴Retd Prof in Microbiology, Sri Padmavati Mahila Visvavidyalayam, Tirupati, 517502, Andhra Pradesh, India
* Corresponding Author : sridevikmam@gmail.com

Received : 27-06-2018     Accepted : 11-07-2018     Published : 15-07-2018
Volume : 10     Issue : 13       Pages : 6552 - 6556
Int J Agr Sci 10.13 (2018):6552-6556

Keywords : Agro waste, Biodegradation, Mango peel, Pectinases, Isolation, Screening, Microorganisms
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to SGS Arts College, Sri Venkateswara University, Tirupati, 517502, Andhra Pradesh, India
Author Contribution : All author equally contributed

As the population and the improved life style of people increasing day by day, results in the increased generation of wastes the country. Especially the food and agricultural industries produce large amount of wastes, causing a serious disposal problem. And it is becoming more problematic in countries like India where the economy is mainly based on agriculture and where the farming practice is very expensive. The past decade, the concept of sustainable development has become entrenched in our social conscience inextricably linking the growth of global economy to the protection of resources and our environment over the other types of waste treatment methods. One of the agro-wastes currently causing pollution problems is the mango peel from fruit processing industries. Microorganisms play an important role in the degradation of pectin which is major component of mango peel. The action of different extracellular enzymes on mango peel leads to the biodegradation of pectin. The type of enzymes is known as pectinases which hydrolyses pectic substances readily. Therefore, the main objective of the present study was to isolate, screen and to study the effect of various factors on pectinolytic activities of Bacillus strain from soils of decomposed fruit waste from mango processing industries.

1. Berardini N., Fezer R., Conrad J., Beifuss U., Carle R. and Schieber A. (2005) J Agri Food Chem., 53,1563-1570.
2. Bayoumi R.A., Yassin H.M., Swelim M.A. and Abdel-All E.Z. (2008) J Appl Sci Res., 4, 1708-1721.
3. Yadav S., Yadav P.K., Yadav D. and Singh Yadava K.D. (2009) Process Biochemistry, 44(1),1-10.
4. Gummadi S.N. and Sunil Kumar (2006) Biochemical Engineering Journal, 30,130–137.
5. Subba Rao N.S. (1999) Soil microorganisms and plant growth. Oxford and IBH Publishing Co., New Delhi, India.
6. Aneja K.R. (2003) Experiments in Microbiology, Plant Pathology and Biotechnology revised fourth edition, New Age International Pvt. Ltd.
7. Fernandes-Salomao T.M., Amorim A.C.R., Chaves-Alves V.M., Coelho J.L.C., Silva D.O., Araújo E.F. (1996) Rev. Microbiol., 27, 15-18.
8. Yin L.J., Huang P.S. and Lin H.H. (2010) Journal of Agricultural and Food Chemistry, 58(17),9833-9837.
9. Andro T., Chambost J.P., Kotoujansky A., Cattaneo J., Bertheau Y., Barras F., Gijsegem F. Van and Coleno A. (1984) J Bacteriol., 160,1199-1203.
10. Punitha A., Basil-Rose M.R., Albino Wins J., Prakash Shoba S. and Mary Mettilda Bai S. (2012) African Journal of Biotechnology, 11(42),10015-10020.
11. Sneath P.A., Mair N.S., Sharpe M.E. and Holts J.G. (1986) Bergey’s Manual of Systematic Bacteriology Vol 2, Baltimore MD: Willam and Wilkins.
12. Mackie and McCartney (2008) Practical Medical Microbiology. (College G, Dugid, JP Fraser, AG and Marmion BP Eds.) 16th Edition. Churchill Livingstone, Edinburgh, London, Melbourne and New York 2, 43-160.
13. Soares M.M.C.N., Da Silva R. and Gomes E. (1999) Rev. Microbial, 30(4), 299-303.