IN VITRO CYTOTOXICITY ANALYSIS OF NITROGEN DOPED GRAPHENE OXIDE HYDROXYAPATITE NANOCOMPOSITE (N2-GO-HA NC) IN CAPRINE WHARTONS JELLY DERIVED MESENCHYMAL STEM CELLS (WJ-MSCs) AND BLOOD CELLS

S.A. DHENGE¹, N.E. GADE²*, O.P. MISHRA³, N. RAWAT⁴, V. SINGH⁵, A. SRIVASTAVA^6
1Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Durg, 491001, India
²Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Durg, 491001, India
³Department of Veterinary Physiology & Biochemistry, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Durg, 491001, India
⁴Department of Veterinary Microbiology, College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Durg, 491001, India
⁵Department of Physics, Banaras Hindu University, Banaras, 221005 India
⁶Department of Physics, Banaras Hindu University, Banaras, 221005 India
* Corresponding Author : nitingadeivri@gmail.com

Received : 11-06-2018     Accepted : 22-06-2018     Published : 30-06-2018
Volume : 10     Issue : 12       Pages : 6387 - 6391
Int J Agr Sci 10.12 (2018):6387-6391

Keywords : WJ-MSCs, blood cells, caprine, nanotoxicity, stem cells
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to College of Veterinary Science & Animal Husbandry, Chhattisgarh Kamdhenu Vishwavidyalaya, Anjora, Durg, 491 001, India. This research was supported by Development grant provided by Agriculture Education Division, ICAR, New Delhi
Author Contribution : All author equally contributed

Present study was designed to analyze in vitro cytotoxicity of different concentrations of Nitrogen dope Graphene Oxide Hydroxyapatite nanocomposites (N2-GO-HA NC) in caprine WJ-MSCs and blood cells. Caprine WJ-MSCs were isolated cultured, characterized and propagated for evaluating nanotoxicity by studying cell morphology, cell viability, growth kinetic, PDT, MTT, hemolysis and CBC assays. Caprine WJ-MSCs after 24 hrs, 48 hrs and 72 hrs exposure with 100 and 50 µg/ml of N2-GO-HA NC showed significant decrease in viable cell number and alterations in cell morphology whereas with 25 and 10 µg/ml of N2-GO-HA NC no significant alteration in cell morphology but significant (P<0.01) increased in cell viability was observed. Similar trend was observed in cell growth kinetics study where significant (P<0.05) decrease in cell growth with increased PDT is estimated in 100 and 50 µg/ml however exposure to 25 and 10 µg/ml doses resulted in significant (P<0.05) increase in growth of caprine WJ-MSCs as compared to control group. MTT assay in caprine WJ-MSCs exposed to 100 and 50 µg/ml of N2-GO-HA NC also revealed significant (P<0.01) decrease in cell number as compared to 25, 10 and 0 µg/ml doses. Hemolysis assay pointed out significant (P<0.01) hemolysis in caprine RBCs exposed to 100 µg/ml dose as compared with 50, 25, 10 and 0 µg/ml doses of N2-GO-HA NC. However, RBCs, WBCs and platelets count were altered non-significantly in 100, 50, 25 and 10µg/ml doses of N2-GO-HA NC as compared to control. The report concludes that higher doses (100 and 50 µg/ml) of N2-GO-HA induce significant toxicity to Caprine WJ-MSCs as well as blood cells (100 µg/ml), however at lower doses (25 and 10 µg/ml) slight growth enhancing effects were observed

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