GENETIC POLYMORPHISMS IN NON-CODING REGIONS OF INSULIN LIKE GROWTH FACTOR-1 GENE IN SIX INDIGENOUS DRAUGHT CATTLE OF INDIA

A. GOGOI¹*, S.M.K. KARTHICKEYAN², P. HEPSHIBA³, N. KUMARAVELU⁴, T. ANNA⁵, K. SENTHILVEL⁶, R. SARAVANAN⁷, P. GANAPATHY^8
1Lakhimpur College of Veterinary Science, Joyhing, North Lakhimpur, 787051, Assam Agricultural University, Jorhat, 785013, Assam, India
²Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
³Madras Veterinary College, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁴Barghur Cattle Research Station, Barghur, Erode, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁵Veterinary College and Research Institute, Tirunelveli, 627358, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁶Veterinary College & Research Institute, Namakkal, 637002, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁷Veterinary College & Research Institute, Namakkal, 637002, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
⁸Barghur Cattle Research Station, Barghur, Erode, Tamil Nadu Veterinary and Animal Sciences University, Chennai, 600051, Tamil Nadu, India
* Corresponding Author : drankita.vet009@gmail.com

Received : 28-05-2018     Accepted : 12-06-2018     Published : 15-06-2018
Volume : 10     Issue : 11       Pages : 6357 - 6360
Int J Agr Sci 10.11 (2018):6357-6360

Keywords : Genetic polymorphism, Draught cattle, Insulin-like growth factor 1 gene, Non-coding region
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to the Indian Council of Agricultural Research (ICAR), New Delhi for the financial assistance provided to the Department of Animal Genetics and Breeding, Madras Veterinary College, Chennai under the scheme ‘Core Laboratory’ functioning through National Bureau of Animal Genetic Resources, Karnal.
Author Contribution : All author equally contributed

This study was undertaken to detect genetic polymorphisms in the non-coding regions of bovine insulin-like growth factor (IGF-1) gene in six indigenous draught cattle breeds viz., Bargur, Hallikar, Kangayam, Ongole, Pulikulum and Umblachery of Southern India. A total of 312 blood samples (52 samples from each breed) were collected and genomic DNA was isolated. Four sets of primers were designed for the amplification of the expressed regions of the IGF-1 gene along with intronic sequences on either side. The presence of eight polymorphisms was detected in the intervening sequences. The variations seen in the intronic regions were at g. 316 C>A (transversion), g. 426 G>A (transition), g. 435 G>A (transition), g. 1747 A>T (transversion), g. 1884 C>A (transversion), g. 1940 C>T (transition), g. 4707 G>A (transition) and g. 4954 C>A (transversion). Deletion of ‘G’ at position g. 4940 and replacement of ‘C’ with ‘A’ at position g. 56413 was found in all the six indigenous breeds. Genotyping these variations in larger number will give significant information on their role among Indian draught cattle.

1. Singh G. (1999) Indian Journal of Animal Sciences, 69, 621-627.
2. Livestock Census Report (2012) 19th Livestock Census. Ministry of Agriculture, New Delhi.
3. Siadkowska E., Zwierzchowski L., Oprządek J., Strzałkowska N., Bagnicka E. and Krzyżewski J. (2006) Animal Science Papers and Reports, 24(3), 225-237.
4. Daughaday W. H. and Rotwein P. (1989) Endocrinology Review, 10, 68-92.
5. Nixon A.J., Brower-Toland B.D. and Sandell L.J. (1999) American Journal of Veterinary Research, 60(10), 1234-1241.
6. Bishop M. D., Tavakkol A., Threadgill D. W., Simmen F. A., Simmen R. C., Davis M. E. and Womack J. E. (1991) Journal of Animal Science, 69, 4306-4311.
7. Miller J.R., Thomsen P.D., Dixon S.C., Tucker E.M., Konfortov B.A. and Harbitz I. (1991) Animal Genetics, 23, 51-58.
8. Kostek M.C., Delmonico M.J., Reichel J.B., Roth S.M., Douglass L., Ferrel R.E. and Hurley B.F. (2005) Journal of Applied Physiology, 98, 2147-2154.
9. Sambrook J.E., Fritsch F. and Maniatis T. (1989) Molecular cloning: A Laboratory Manual, 2nd Ed. Cold Spring Harbor Laboratory Press, New York, USA.
10. Zhang Y., Du H., Chen J. and Yang G. (2008) Journal of Genetics and Genomics, 35, 163-169.
11. Chung E. R. and Kim W.T. (2005) Asian-Australasian Journal of Animal Sciences, 18(8), 1061-1065.
12. Gogoi A., Karthickeyan S.M.K. and Chabukdhara P. (2016) Journal of Livestock Biodiversity, 6(2), 82-86.