BIOCHEMICAL INDICES AND WOUND MORPHOMETRY IN RABBITS ON EXCISIONAL FULL THICKNESS SKIN WOUND HEALING EFFICACY BY USING DIFFERENT EXTRACT OF Salix acmophylla LEAVES OINTMENT

QUMAILA SAKEENA¹*, MD MOIN ANSARI², SADAF SAKEEN³, D.M. MAKHDOOMI⁴, T. IMTIYAZ^5
1Division of Veterinary Surgery and Radiology, FVSc and AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, 190025, India
²Division of Veterinary Surgery and Radiology, FVSc and AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, 190025, India
³Division of Veterinary Gynaecology and Obstruction, FVSc and AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, 190025
⁴Division of Veterinary Surgery and Radiology, FVSc and AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, 190025, India
⁵Division of Veterinary Surgery and Radiology, FVSc and AH, Sher-e-Kashmir University of Agricultural Sciences and Technology of Kashmir, Shalimar, 190025, India
* Corresponding Author : qumaila.sakeena123@gmail.com

Received : 15-05-2018     Accepted : 25-05-2018     Published : 30-05-2018
Volume : 10     Issue : 10       Pages : 6091 - 6095
Int J Agr Sci 10.10 (2018):6091-6095

Keywords : Atomic Absorption Spectroscopy, Bacterial Colony Count, Copper, Zinc 5% Aqueous extract, 5% Ethanolic extract
Academic Editor : Dr Amrita A Vasava, Aithal H.P.
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to Sher-e-Kashmir University of Agricultural Sciences and Technology, Shalimar, Kashmir,190025, India
Author Contribution : All author equally contributed

The present study was aimed to estimate the concentration of Glucose (md/dl) using Accu-Check Active Glucose meter, Zinc (µg/ml of serum) and Copper (µg/ml of serum) using atomic absorption spectroscopy method at 213.9 nm and 324.8 nm respectively and estimating the Bacterial colony count by taking the wound washings in rabbits after creation of 72 excisional full thickness skin wound and post treatment with the 5% ethanolic and 5% aqueous extract ointment group (6 rabbits each) and comparing the results with the control group (6 rabbits). The lowest concentration of Glucose and Bacterial count and highest concentration of trace elements i.e., Zinc and Copper was seen in 5% ethanolic extract ointment treated group. The wounds of 5% Ethanolic extract ointment of Salix treated groups evinced complete healing (100% contraction) which was followed closely by 5% aqueous extract ointment of Salix acmophylla and distantly by normal saline treated group on day 21 post-wounding, where mean wound contraction /size of 100%. 99.98% and 96.24% respectively was recorded.

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