ANTIOXIDANT ANALYSIS OF ESSENTIAL OILS AND METHANOLIC EXTRACTS OF Artemisia vulgaris

KUNAL MADHAV¹, ZAFAR HAIDER², UJJWAL BHANDARI³, PRATIBHA⁴, GAURAV NAIK⁵*
¹Centre for Aromatic Plants, Selakui, 248011, Uttarakhand
²Centre for Aromatic Plants, Selakui, 248011, Uttarakhand
³Centre for Aromatic Plants, Selakui, 248011, Uttarakhand
⁴Centre for Aromatic Plants, Selakui, 248011, Uttarakhand
⁵Centre for Aromatic Plants, Selakui, 248011, Uttarakhand
* Corresponding Author : 123.gaurav@gmail.com

Received : 19-03-2018     Accepted : 11-04-2018     Published : 15-04-2018
Volume : 10     Issue : 7       Pages : 5710 - 5713
Int J Agr Sci 10.7 (2018):5710-5713

Keywords : Essential Oils, Anti-Oxidant, DPPH, TFC, TPC
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to NMHS (National Mission on Himalayan Studies) for financial assistance in the form of JRF. Author thankful to Dr. Nirpendra Chauhan (Scientist-in-Charge, CAP) for permitting to utilise the facilities. The authors also extend a warm and cordial thanks to Anuj Pundir, for the technical support.
Author Contribution : All author equally contributed

Recent researches have focused on the isolation and identification of novel compounds which has strong biological activities. Anti-oxidant analysis is one of the several activities which has gained interest of many researchers and many plant species have been used for the analysis of this property. The present study focuses on the comparative analysis of Anti-oxidant properties of Methanolic Extracts and Essential oils of Artemisia vulgaris, a plant belonging to Asteraceae family. GC-MS study of the essential oils of this plant showed that the major phyto-constituents were (10.7%) followed by ß endesmol (8.95%). DPPH radical scavenging assay was used for the analysis of Antioxidant activity and it was found that the Methanolic extract had the maximum activity of 72.65% while the Essential oil had 88.65% of the activity. The TPC content of the Methanolic extracts was found to be 347.69 mg GAE/g and the TFC content was found to be 101.17 mg GAE/g respectively.

1. Anwar F., Ahmad Naveed, Alkharfy, Khalid M.& Gilani A. (2016) Mugwort ( Artemisia vulgaris ) Oils Author ’ s personal copy. In Essential Oils in Food Preservation, Flavour and Safety.
2. Baykan Erel Ş., Reznicek G., Şenol S. G., Karabay Yavaşoǧulu N. Ü., Konyalioǧlu S. & Zeybek A. U. (2012) Turkish Journal of Biology, 36(1), 75–84.
3. Bharati K. A. & Sharma B. L. (2012) Ethnobotany Research and Applications, 10, 339–356.
4. Bhatt Ranjan L., Lee Sug J. & Baek Hwa S. (2007) Koren Journal of Oriental Physiology and Pathology, 21(2), 528–531.
5. Blagojević P., Radulović N., Palić R. & Stojanović G. (2006) Journal of Agricultural and Food Chemistry, 54(13), 4780–4789.
6. Ghimire B. K., Seong E. S., Kim E. H., Ghimeray A. K., Yu C. Y., Ghimire B. K. & Chung I. M. (2011) Journal of Medicinal Plants Research, 5(10), 1884–1891.
7. Ghosh T., Mitra P. & Mitra P. K. (2013) International Journal of Herbal Medicine Effect, 1(2), 30–34.
8. Haider F., Dwivedi P. D., Naqvi A. A. & Bagchi G. D. (2003) Journal of Essential Oil Research, 15(6), 376–378.
9. Ilahi I. & and Farman Ullah (2013) International Journal of Innovation and Applied Studies, 2(2), 189–195.
10. Judžentien A., & Buzelyte J. (2006) Chemija, 17(1), 12–15.
11. Karabegović I., Nikolova M., Veličković D., Stojičević S., Veljković V. & Lazić M. (2011) Chinese Journal of Chemical Engineering, 19(3), 504–511.
12. Khan A. ullah, & Gilani A. H. (2009) Journal of Ethnopharmacology, 126(3), 480–486.
13. Lohani H., Gwari G., Bhandari U., Haider S. Z., Andola H. & Chauhan N. (2016) Journal of Essential Oil Bearing Plants, 19(1), 103–107.
14. Nikolova M. (2011) Pharmacognosy Research, 3(4), 256–259.
15. Shah N. C. (2014) The Scitech Journal, 1(1), 29–38.
16. Siddiqui N., Rauf A., Latif A. & Mahmood Z. (2017) Journal of Taibah University Medical Sciences, 12(4), 360–363.
17. Sil P., Thaku, S. & Ghosh P. (1987) Journal of Chromatography, 403, 285–287.
18. Singh B. R., Singh V., Singh R. K., Toppo S., Haque N. & Ebibeni N. (2012) Medicinal Plants, 4(2), 76–82.
19. Wani H., Shah S. & Banday J. (2014) The Journal of Phytopharmacology, 3(2), 90–94.
20. Wojdyło A., Oszmiański J. & Czemerys R. (2007) Food Chemistry, 105(3), 940–949.