PHYSIOLOGICAL AND GENETIC DIVERSITY ANALYSIS OF ADVANCED BREEDING LINES OF CLUSTER BEAN [Cyamopsis tetragonoloba L. Taub] FOR DROUGHT TOLERANCE

K. RASHMI¹, S. MOHAN KUMAR²*
¹Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
²Department of Biotechnology and Crop Improvement, College of Horticulture, University of Horticultural Sciences, GKVK, Bengaluru, 560065, Karnataka, India
* Corresponding Author : mohancph@rediffmail.com

Received : 23-03-2018     Accepted : 28-03-2018     Published : 30-03-2018
Volume : 10     Issue : 6       Pages : 5548 - 5550
Int J Agr Sci 10.6 (2018):5548-5550

Keywords : Cyamopsis tetragonoloba, RWC, Genetic diversity, Dendrogram, SSR analysis
Academic Editor : Anilkumar C
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
Author Contribution : All author equally contributed

An experiment was carried out in College of Horticulture, UHS campus, Bengaluru during 2016-17. Experiment was laid out in Complete Randomized Design (CRD) comprising of 19 treatments with 2 replications. The present study was undertaken to evaluate the physiological variability for drought tolerance and genetic diversity among nineteen advanced breeding lines by using SSR marker. Nineteen advanced breeding lines including the check variety Pusanavabahar were raised in pots containing soil, sand and fertilizers in the ratio 1:2:1 and screened for relative water content (RWC), which is the most appropriate estimate of plant water status in terms of physiological consequence of cellular water deficit 35 DAS under drought. Maximum per cent of RWC was recorded in PusaNavabahar (83.34%) followed by the line COHBCB 41 (85.12%). Molecular markers are being widely used in various areas of plant breeding as an important tool for evaluating genetic diversity and determining cultivars identity. A dendrogram constructed based on unweighed pair group method of arithmetic means (UPGMA) using data derived from 22 SSRs grouped the 19 cluster bean advanced breeding lines into two main clusters with 0.62 similarity. The ranges of dissimilarity among the cluster beans of advanced breeding lines were varied from 0.62 to 1.00.

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