MOLECULAR IDENTIFICATION OF TERMITOMYCES SPECIES FROM WESTERN GHATS OF KARNATAKA

D.B. SANTHOSH¹*, A.N. RAMESH², D. AKASH³, M.P. BRIJESH PATIL⁴, N. EARANNA^5
1Department of Plant Biotechnology, University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
²Department of Plant Biotechnology, University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
³Department of Plant Biotechnology, University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
⁴Department of Plant Biotechnology, University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
⁵Department of Plant Biotechnology, University of Agricultural sciences, Bengaluru, 560065, Karnataka, India
* Corresponding Author : santhuroyal99@gmail.com

Received : 23-03-2018     Accepted : 28-03-2018     Published : 30-03-2018
Volume : 10     Issue : 6       Pages : 5551 - 5553
Int J Agr Sci 10.6 (2018):5551-5553

Keywords : Western Ghats, Mushroom, ITS, Molecular identification, Genus Termitomyces
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to University of Agricultural sciences, Bengaluru, 560065, Karnataka, India. Author thankful to Department of Biotechnology (DBT), Govt of India for financial support for this research
Author Contribution : All author equally contributed

Western Ghats of Karnataka is one of the richest biodiversity hotspot in India. In the present study, mushroom species in the Genus Termitomyces, collected from Shivamogha region of Western Ghats during monsoon season (June-September) with the help of Siddi and Adivasi tribal community. During collection, the field information was recorded and the samples were designated as Sample -1, Sample -2, Sample -3 and Sample -4. Further, these mushrooms were identified by internal transcribed spacer (ITS) region sequence homology using NCBI data base. The mushrooms identified based on sequence homology are Termitomyces micro carpus (Sample-1), Termitomyces Sp (Sample-2), Termitomyces Sp (Sample-3) and Termitomyces Sp (Sample-4). All are edible mushrooms belong to Genus Termitomyces.

1. Froslev T.G., Aanen D.K., Laessoe T. and Rosendahl S. (2003) Mycological Research, 107, 1277–1286.
2. Aanen D.K. and Eggleton P. (2006) Current Biology, 15, 851−855.
3. Tang B.H., Wei T.Z., and Yao Y.J. (2006) Mycotaxon., 95, 285–293.
4. Rajaratnam S. and Thiagarajan T. (2012) Eur. J Biol, 2(2), 369-373.
5. Oyetayo V. O. (2012) Sci. World J., 1, 1-5.
6. Sambrook J., Fritsch F. F. and Maniatis T. (1989) Cold Spring Harbor, NY, pp.931-957.
7. Altschul S.F., Gish W., Miller W., Myers E.W. and Lipman D. J. (1990) J. Mol. Biol., 215, 403-410.
8. Rai R.D. (1997) Medicinal mushroom: In: Advances in mushroom biology and production. Mushroom Society of India. NRCM, Solan HP., 355-368
9. Prakasam V., Karthikayani B.A., Thiribhuvanamala G.U., Chandrasekar G., Veeralakshmi S.U., Ahila P., Sakthivel K. and Malarkodi B. (2011) Tricholoma giganteum-a new tropical edible mushroom for commercial cultivation in India. In: Proceedings of the 7th International Conference on Mushroom Biology and Mushroom Products (ICMBMP7), 438-445.
10. Rouland Lefevre C.R., Diouf M.N., Brauman A. and Neyra M. (2002) Molecular Phylogenetics and Evolution., 22, 423–429.
11. Nobre T. and Aanen D. K. (2012) Insects, 307–323.