EFFECT OF ACEPHATE PESTICIDE ON MEIOSIS IN ONION (Allium cepa L.)

ALOKESH DAS¹, SAMRAT DUTTA², SUDIPA NAG³, SUBRATA MANDAL⁴*
¹Department of Botany, Rampurhat College, Rampurhat, Birbhum, 731224, University of Burdwan, Bardhaman, 713104, West Bengal, India
²Department of Botany, Rampurhat College, Rampurhat, Birbhum, 731224, University of Burdwan, Bardhaman, 713104, West Bengal, India
³Department of Botany, Rampurhat College, Rampurhat, Birbhum, 731224, University of Burdwan, Bardhaman, 713104, West Bengal, India
⁴Department of Agronomy, Rathindra Krishi Vigyan Kendra, Institute of Agriculture Visva-Bharati, Sriniketan, Birbhum, 731224, West Bengal, India
* Corresponding Author : smkvkvb@gmail.com

Received : 15-03-2018     Accepted : 20-03-2018     Published : 30-03-2018
Volume : 10     Issue : 6       Pages : 5456 - 5459
Int J Agr Sci 10.6 (2018):5456-5459

Keywords : Allium cepa L., acephate, chromosomal aberrations, pesticide, genetic damage
Academic Editor : Meignanalakshmi Sundaram
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Department of Botany, Rampurhat College, Rampurhat, Birbhum, 731224 for carry out the experiment in the Laboratory. Author thankful to Rathindra Krishi Vigyan Kendra, Institute of Agriculture Visva-Bharati, Sriniketan, Birbhum, 731224, West Bengal for supplying the pesticides, onion, etc., for the experiment
Author Contribution : All author equally contributed

The effect of acephate (pesticide) on the meiosis of Allium cepa L. was assayed for detecting the mutagenic behavior of this chemical. Onion flowers were treated with 0.750 g /lt (general recommendation for farmers use) water and 0.375 g/ lt (50% of general recommendation) with acephate at 24 hrs and 48 hrs duration and distilled water as control. Specific types of chromosomal aberrations have been induced by acephate. The result showed that acephate induced meiotic chromosomal aberrations like chromosome stickiness and clumping, c-meiosis, multipolarity, bridges, laggards, asynchrony, absence of cytokinesis, micronuclei, triads and diads were found more at 0.750 g/lt and 0.375 g/lt of water concentrations with different time of exposure. Frequently, the chromosomal aberrations are found identical in specific phases of meiosis in Allium cepa L. anther cells. It may be stated that the chromosomal aberrations due to the application of acephate pesticide in Allium cepa L. may be used as important test battery to detect the genetic damage.

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