POTASSIUM AND ZINC SOLUBILIZING EFFICIENCY ASSESSMENT OF MICROORGANISMS FROM DIFFERENT RHIZOSPHERE SOILS

BHAGWAN SINGH DHAKED¹*, S. TRIVENI², R. Subhash Reddy³, G. PADMAJA⁴, AJAY KUMAR⁵, AMAN JAISWAL⁶, DEEPAK KUMAR KOLI^7
1Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
²Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
³Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
⁴Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana, India
⁵Division of Microbiology, ICAR-Indian agricultural Research Institute, New Delhi, 110012
⁶Division of Microbiology, ICAR-Indian agricultural Research Institute, New Delhi, 110012
⁷Division of Microbiology, ICAR-Indian agricultural Research Institute, New Delhi, 110012
* Corresponding Author : jangraajay8888@gmail.com

Received : 16-03-2018     Accepted : 20-03-2018     Published : 30-03-2018
Volume : 10     Issue : 6       Pages : 5437 - 5442
Int J Agr Sci 10.6 (2018):5437-5442

Keywords : Potassium and zinc solubilizing bacteria, potassium alumino silicate
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to The Head and Advisory Committee members, Department of Agricultural Microbiology & Bioenergy, College of Agriculture, Professor Jayashankar Telangana State Agricultural University, Rajendranagar, Hyderabad, 500030, Telangana
Author Contribution : All author equally contributed

The amount of K released from potassium alumino silicate in a broth by the KSB isolates at 7, 15, 21 days after incubation (DAI) were increased with increase in incubation time and was maximum at 20 DAI (6.5 to 73.4 (μg/ml). Among the isolates KSB - 2 released maximum amount of K from potassium alumino silicate (73.4 (μg/ml). The KSB-2 significantly produced higher bacterial count (47.0 x 10 8 cfu ml-1). The amount of polysaccharide production was observed on glucose minimal agar medium and visually scored. Compared to all, KSB-2 isolate was best performer (+++) followed by KSB-4 was a moderate producer. The amount of Zn available in broth at 4th, 8th and 16th day of incubation in ZnO and Zinc phosphate supplemented growth medium increased with increase in incubation time and was maximum at 16 DAI which was 3.98 to 36.62 μg/ml for ZnO wih pH change of 7.12 to 3.96 and 3.20 to 32.25 μg/ml for Zinc phosphate with pH change 7.0 to 4.9. The zinc solubilizing bacterium ZnSB-2 (36.62 μg/ml) showed the maximum value of available zinc in broth at 4th, 8th and 16th day of incubation in ZnO supplemented growth medium. The zinc solubilizing bacterium ZnSB-8 (32.25 μg/ml) showed the maximum value of available zinc in broth at 4th, 8th and 16th day of incubation in Zinc phosphate supplemented growth medium. The ZnSB - 5 significantly produced higher bacterial counts (2.55 x 10 8 cfu ml-1). The amount of Zn available in broth for ZnSF at 4th, 8th and 16th day of incubation in ZnO supplemented growth medium increased with increase in incubation time and was maximum at 16 DAI which was 6.32 to 20.28 μg/ml for ZnO with pH change of 6.60 to 4.30. The zinc solubilizing fungi ZnSF-1 (20.28 μg/ml) showed the maximum value of available zinc in broth at 4th, 8th and 16th day of incubation in ZnO supplemented growth medium. ZnSF-1 significantly produced higher count (123 x 10 6 cfu ml -1) followed by ZnSF-2 (119.0 x 10 6 cfu ml-1). The bacterial isolate KSB-2 solubilized more potassium, ZnSB-8 solubilized more zinc phosphate and ZnSF-1 solubilized more zinc oxide in broth assay. and ZnSB-2 in broth assay. The solubilization of insoluble source in liquid culture broth increased with increase in incubation time. The isolates produced exopolysaccharide and organic acids for solubilization.

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