MICROSATELLITE MARKER ASSISTED SELECTION AND GENERATION OF F1 HYBRIDS FOR RICE BIOFORTIFICATION

BASANTI BRAR¹*, DEEPIKA CHAUDHARY², R.K. JAIN³, SUNITA JAIN^4
1Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana
²Chaudhary Devi Lal University, Sirsa, 125055, Haryana
³Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana
⁴Department of Molecular Biology, Biotechnology and Bioinformatics, Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana
* Corresponding Author : basantibrar@gmail.com

Received : 17-03-2018     Accepted : 20-03-2018     Published : 30-03-2018
Volume : 10     Issue : 6       Pages : 5427 - 5430
Int J Agr Sci 10.6 (2018):5427-5430

Keywords : Enhancement, mineral content Micronutrient deficiency, rice and zinc
Conflict of Interest : None declared
Acknowledgements/Funding : Author thankful to Chaudhary Charan Singh Haryana Agricultural University, Hisar, 125004, Haryana and Chaudhary Devi Lal University, Sirsa, 125055, Haryana
Author Contribution : All author equally contributed

Biofortification is an approach for enhancement of the micronutrient content of staple crops. This possible because only due to the lots of genetic variation exist within the genome of staple food crops like rice. Greater than 2 billion persons of the humanity are micronutrient- iron as well as zinc deficient. Rice (Oryza sativa L.) is the major source of food for more than half of the world’s population. As a model cereal crop, the complete genome sequences of rice has become fundamental tool for study gene functions and correlate it with the practical applications in plants. At present, rice researchers devote much effort to generating mineral rich rice genotypes to combact the micronutrient malnutrition. Molecular analysis, genetic transformation and molecular breeding combine with mineral content examination for presented rice germplasm. In the present study we report the F1 identification of rice plant after crossing the high yielding genotypes with micronutrient (iron and Zinc) rich genotypes using microsatellite markers both by agarose as well as polyacrylamide gel electrophoresis.

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