ISSN : 0975-3710
EISSN : 0975-9107
JITENDRA KUMAR1*, R. RABINDRAN2
1Department of Plant Pathology, Centerfor Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
2Department of Plant Pathology, Centerfor Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu, India
* Corresponding Author : jitpatho@gmail.com
Received : 25-02-2018 Accepted : 02-03-2018 Published : 15-03-2018
Volume : 10 Issue : 5 Pages : 5255 - 5259
Int J Agr Sci 10.5 (2018):5255-5259
Keywords : Cassava, Gemini viruses, ICMV, Multiplex PCR, SLCMV
Conflict of Interest : None declared
Acknowledgements/Funding : The author is grateful to I.C.A.R., Ministry of Agriculture, Government of India for providing Junior Research Fellowship (JRF) that helped me lot during my research. We are also grateful to the Professor and head, Department of Plant Pathology, Centre for Plant Protection Studies, Tamil Nadu Agricultural University, Coimbatore, 641003, Tamil Nadu for providing the laboratory facilities
Author Contribution : All author equally contributed
Cassava mosaic disease (CMD) caused by cassava mosaic Gemini viruses (CMGs) (Geminiviridae: Begomovirus) is undoubtedly the most important constraint to the production of cassava in South India. Studies were conducted for identification of cassava mosaic virus at Department of Plant Pathology, TNAU, Coimbatore during 2008-09 and 2009-10. Diseased samples were collected from different places of Tamil Nadu and analyzed for the presence of geminiviruses by PCR using Deng’s degenerate primers. Sri Lankan cassava mosaic virus (SLCMV) detected by using TAS-ELISA and multiplex PCR using to differentiate SLCMV and ICMV if dual infection is present. Monoclonal antibody AS-0424/1 (DSMZ) was used in TAS-ELISA. Among all the samples tested, highest OD value (0.941) was recorded in samples collected from Pollachi of Coimbatore district and lowest OD value (0.247) was recorded from the samples collected from Ambilikkai of Dindigul district. PCR products by using Deng’s degenerate primers were amplified at 560 bp from all cassava samples, revealed the association of Geminiviruses with this disease. The multiplex PCR analysis revealed the presence of only SLCMV (599 bp band size). In all the samples tested neither ICMV nor dual occurrence of both ICMV and SLCMV were observed.
1. Abraham A. (1956) Tapioca cultivation in India. Farm Bulletin No. 17. Indian Council of Agricultural Research, New Delhi, 20.
2. Adhikary N.K., Kumar M. and Tarafdar J. (2018) International Journal Current Microbiology and Applied Science 7(1), 2887-2893
3. Alagianagalingam M.N. and Ramakrishnan K. (1966) South Indian Horticulture, 14(4), 71–72.
4. Austin M.D.N. (1986) Asian Agribusiness, 22(3): 10-14.
5. Bock K.R. and Harrison B.D. (1985) Descriptions of Plant Viruses, 37(2), 297-305.
6. Bock K.R., Guthrie E.J. and Figueiredo G. (1981) Annals of Applied Biology, 99(4), 151-159.
7. Clark M.F. and Adams A.N. (1977) Journal of General Virology,
34(3), 475-483.
8. Dasgupta I., Malathi V.G. and Mukherjee S.K. (2003) Current Science, 84(3), 340–354.
9. Deng D., McGrath P.F., Robinson D.J. and Harrison B.D. (1994) Annals of Applied Biology, 125(3), 327-336.
10. Duraisamy R., Arumugam C., Natesan s., Muthurajan R., Gandhi K., Lakshmanan P., Janavi G. J., Karuppusamy N. and Chokkappan M. (2017) International Journal of Current Microbiology and Applied Sciences, 6(7), 1305-1317.
11. Harrison B.D., Swanson M.M., McGrath P.F. and Fargette D. (1991) Report of the Scottish Crop Research Institute, 1990, 88–90.
12. Jos J.S., Bai K.V. and Nair N.G. (1984) Indian Phytopathology, 37(3), 696-699.
13. Lodhi M.A., Ye G.N., Weeden N.F. and Reisch B. (1994) Plant Molecular Biology Reporter, 12(4), 6-13.
14. Malathi V.G. and Sreenivasan M.A. (1983) Journal of Root Crops, 9(2), 69-73.
15. Ndunguru J., Legg J.P., Aveling T.A.S., Thompson G.A., and Fauquet C.M. (2005) Virology Journal, 2(1), 321- 328.
16. Patil B.L., Rajasubramaniam S., Bagchi C. and Dasgupta I. (2005) Archives of Virology, 150(2), 389-397.
17. Rajinimala N., Rabindran R. and Mohan S. (2007) Studies on molecular variability of Cassava infecting geminiviruses (CIGs) in Tamil Nadu and its ecofriendly management. Ph.D. Thesis. Tamil Nadu Agricultural University, Coimbatore, 167.
18. Thankappan M. and Chacko C.I. (1976) Journal of Root Crops, 16(2), 45-47.