DETECTION OF AVIAN INFECTIOUS BRONCHITIS VIRUS BY RT-PCR AND RT-LAMP

AJAY DANDOTIYA¹, GURPREET KAUR²*, SREEKALA S. MOHANDAS³, P.N. DWIVEDI^4
1Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India
²Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India
³Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India
⁴Department of Veterinary Microbiology, College of Veterinary Science, Guru Angad Dev Veterinary and Animal Sciences University, Ludhiana-141001, Punjab, India
* Corresponding Author : gurpreet7502@rediffmail.com

Received : 31-07-2017     Accepted : 05-08-2017     Published : 24-08-2017
Volume : 9     Issue : 39       Pages : 4594 - 4597
Int J Agr Sci 9.39 (2017):4594-4597

Keywords : Poultry, IB virus, viral RNA, RT-PCR, RT-LAMP
Academic Editor : Dr J Ramachandran, Monoj Sutradhar
Conflict of Interest : None declared
Acknowledgements/Funding : The authors are thankful to the Director of Research, GADVASU, Ludhiana for providing the necessary research facilities
Author Contribution : P N Dwivedi and Gurpreet Kaur designed the study. Ajay Dandotiya, Sreekala S. Mohandas and Gurpreet Kaur carried out the experiments. Ajay Dandotiya, Gurpreet kaur and P N Dwivedi analysed the results. All the authors drafted the manuscript

Infectious bronchitis (IB) is caused by infectious bronchitis virus (IBV) belonging to family Coronaviridae. It is a highly contagious disease of poultry and is common throughout the world where poultry are produced commercially. PCR on reverse transcribed RNA is a potent technique for the detection of IBV. Tissue samples of Trachea, Kidney, Lung and Oviduct were collected from fifty layer birds and these were used for RNA extraction. The primers included S2F3 and S2B3 that amplify all IBV serotypes. The results of this study showed that 14% of the samples were positive to IBV by RT-PCR and RT-LAMP was optimized for further detection of IB virus.

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