ISOLATION AND SCREENING OF INDUSTRIALLY IMPORTANT POLYGALCTURONASE PRODUCING FUNGI FROM THE MANGROVE SOILS OF KRISHNA DISTRICT ANDHRA PRADESH

K. RAVI¹, M. RAGHU RAM²*
¹Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510, India
²Department of Botany and Microbiology, Acharya Nagarjuna University, Nagarjuna Nagar, Guntur, Andhra Pradesh 522510, India
* Corresponding Author : mraghuram2002@gmail.com

Received : 21-03-2017     Accepted : 04-04-2017     Published : 24-04-2017
Volume : 9     Issue : 19       Pages : 4193 - 4195
Int J Agr Sci 9.19 (2017):4193-4195

Keywords : Polygalacturonase, Pectin Agar Medium (PAM), zone of hydrolysis
Academic Editor : D. T. Meshram
Conflict of Interest : None declared
Acknowledgements/Funding : Author would like to acknowledge the department of Botany and Microbiology, Acharya Nagarjuna university, Guntur and also thankful to UGC- RGNF for providing financial support for this work
Author Contribution : None declared

For the present study, soil samples were collected from mangrove areas of Gilakaladindi and Malakayalanka of Krishna District. Soil samples were serially diluted and plated on pectin agar plates for isolation of Fungi. The isolates were identified as Penicillium citrinum RR 101(KU 613360) and Aspergillus oryzae RR 103(KU 613361) Penicillium griseofulvum RR104 (KU613362) and Aspergillus sp RR102. Qualitative assay of exo-polygalacturonase of fungal isolates was carried out by growing them on to the pectin agar plates and the zone of hydrolysis around the fungal colonies was measured after flooded with iodine solution. Aspergillus sp. RR 102 showed the maximum zone of hydrolysis (14 mm) after 192 h at 370C. Maximum Polygalacturonase enzyme activity was recorded in Aspergillus sp RR 102 (2.40 IU/ml).

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