BIOLOGICAL INDEXING OF PAPAYA RING SPOT VIRUS (PRSV) in Carica papaya

DEEPA NAVANATH MUSKE¹*, ANITHA PETER², S.J. GAHUKAR³, AMRAPALI A. AKHARE^4
1Biotechnology Center, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444104, Maharashtra
²Department of Plant Biotechnology, University of Agricultural Sciences, GKVK, Bangalore, 560065
³Biotechnology Center, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444104, Maharashtra
⁴Biotechnology Center, Dr Panjabrao Deshmukh Krishi Vidyapeeth, Akola, 444104, Maharashtra
* Corresponding Author : muskedeepa@gmail.com

Received : 21-02-2016     Accepted : 12-01-2017     Published : 24-01-2017
Volume : 9     Issue : 4       Pages : 3728 - 3730
Int J Agr Sci 9.4 (2017):3728-3730

Keywords : Zucchini, PRSV(Papaya Ring spot Virus), Bioassay, ELISA, PCR, Indexing
Academic Editor : Dulangi Karunarathna, Nabanita Chakraborty
Conflict of Interest : None declared
Acknowledgements/Funding : The authors thank DBT, Government of India, New Delhi, for the funding under its HRD program
Author Contribution : None declared

India holds the forth position in the world in producing Papaya (56, 39,000 metric tons in 2014). The production has lowers not increased in proportion in the area because of severe infection of PRSV which causes 60 to 100 present losses in the field. Which is responsible for not only reduced in production also quality of fruit is deteriorated and market value has been lowered. The export of papaya and its related products has been stopped because of PRSV infection. So, the present investigation was carried out to confirm presence of virus in infected sample without any advanced technology and skilled techniques with short period of time. There are ample of techniques were developed still but they are supported with sophisticated instrumentation and costly chemicals and these are having hazardous effect on workers. So in these work the presence of virus was tested by using indicator plant i.e. live plant samples of summer squash. Zucchini (summer squash) which is indicator plant of PRSV used for the bio-assay. After infection of virus to the four leaf seedlings it was found that after manual infection in controlled house inoculated plants shows symptoms like dark green spots accumulated on leaf surface after one week. In this way within a week, we can confirm presence of virus in a seedling material which is major carrier of PRSV from nursery to field.